PA5-34801

antibody from Invitrogen Antibodies

Targeting: IL21 IL-21, Za11

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34801

Provider

Invitrogen Antibodies

Product name

IL-21 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HL-60. Predicted reactivity: Dog (80%), Pig (80%), Sheep (82%), Rhesus Monkey (96%), Bovine (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.68 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC).

Hui Z, Zhang J, Ren Y, Li X, Yan C, Yu W, Wang T, Xiao S, Chen Y, Zhang R, Wei F, You J, Ren X
Cell death & disease 2022 Jul 13;13(7):607

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Adenovirus-mediated overexpression of interleukin-21 regulates the development of oral squamous cell carcinoma in vitro.

Liu H, Liu P, Sun D, Xing D, Wang X, Yang J, Wang S
Oncology letters 2020 Sep;20(3):3006-3014

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Expression of the interleukin-21 and phosphorylated extracellular signal regulated kinase 1/2 in Kimura disease.

Chen QL, Li CX, Shao B, Gong ZC, Liu H, Ling B, Abasi K, Hu LL, Wang B, Yin XP
Journal of clinical pathology 2017 Aug;70(8):684-689

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IL21 using 30 µg of HL-60 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an IL21 polyclonal antibody (Product # PA5-34801) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IL-21 Polyclonal Antibody (Product # PA5-34801). Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-21 Polyclonal Antibody (Product # PA5-34801) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Expression levels of IL-21 in OSCC tissues. (A) mRNA expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using reverse transcription-quantitative PCR. (B) Protein expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using western blotting. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data are presented as the mean +- SEM from three independent experiments. **P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Adenoviral-mediated overexpression of IL-21 in CAL-27 cells. (A) Reverse transcription-quantitative PCR or (B) western blotting was used to analyze the mRNA or protein expression levels, respectively, of IL-21 in CAL-27 cells infected with Ad- or Ad-IL-21. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data were expressed as the mean +- SEM from three independent experiments. *P