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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-34801 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-21 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HL-60.
- Concentration
- 0.68 mg/mL
Submitted references Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC).
Adenovirus-mediated overexpression of interleukin-21 regulates the development of oral squamous cell carcinoma in vitro.
Expression of the interleukin-21 and phosphorylated extracellular signal regulated kinase 1/2 in Kimura disease.
Hui Z, Zhang J, Ren Y, Li X, Yan C, Yu W, Wang T, Xiao S, Chen Y, Zhang R, Wei F, You J, Ren X
Cell death & disease 2022 Jul 13;13(7):607
Cell death & disease 2022 Jul 13;13(7):607
Adenovirus-mediated overexpression of interleukin-21 regulates the development of oral squamous cell carcinoma in vitro.
Liu H, Liu P, Sun D, Xing D, Wang X, Yang J, Wang S
Oncology letters 2020 Sep;20(3):3006-3014
Oncology letters 2020 Sep;20(3):3006-3014
Expression of the interleukin-21 and phosphorylated extracellular signal regulated kinase 1/2 in Kimura disease.
Chen QL, Li CX, Shao B, Gong ZC, Liu H, Ling B, Abasi K, Hu LL, Wang B, Yin XP
Journal of clinical pathology 2017 Aug;70(8):684-689
Journal of clinical pathology 2017 Aug;70(8):684-689
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL21 using 30 µg of HL-60 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an IL21 polyclonal antibody (Product # PA5-34801) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IL-21 Polyclonal Antibody (Product # PA5-34801). Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-21 Polyclonal Antibody (Product # PA5-34801) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL-21 Polyclonal Antibody detects IL21 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. IL-21 Polyclonal Antibody (Product # PA5-34801) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Expression levels of IL-21 in OSCC tissues. (A) mRNA expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using reverse transcription-quantitative PCR. (B) Protein expression levels of IL-21 in OSCC tissues and adjacent normal tissues were detected using western blotting. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data are presented as the mean +- SEM from three independent experiments. **P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Adenoviral-mediated overexpression of IL-21 in CAL-27 cells. (A) Reverse transcription-quantitative PCR or (B) western blotting was used to analyze the mRNA or protein expression levels, respectively, of IL-21 in CAL-27 cells infected with Ad- or Ad-IL-21. (C) Semi-quantification of the western blotting data presented in part B. Expression levels of IL-21 were normalized to beta-actin. The data were expressed as the mean +- SEM from three independent experiments. *P
