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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC594P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-21 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-21 in Western blots. Does not cross-react with recombinant human IL-21, recombinant mouse (rm) IL-15, or rmIL-12.
- Reactivity
- Mouse
- Host
- Rat
- Antigen sequence
Q9ES17- Isotype
- IgG
- Antibody clone number
- 149204
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Enhanced GITR/GITRL interactions augment IL-27 expression and induce IL-10-producing Tr-1 like cells.
Carrier Y, Whitters MJ, Miyashiro JS, LaBranche TP, Ramon HE, Benoit SE, Ryan MS, Keegan SP, Guay H, Douhan J, Collins M, Dunussi-Joannopoulos K, Medley QG
European journal of immunology 2012 Jun;42(6):1393-404
European journal of immunology 2012 Jun;42(6):1393-404
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-21 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (light orange filled histogram) or treated with PMA and Calcium Ionomycin (dark orange filled histogram) were stained with Rat Anti-Mouse IL-21 PE-conjugated Monoclonal Antibody (Catalog # IC594P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
