MAB594

antibody from R&D Systems

Targeting: IL21 IL-21, Za11

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB594

Provider

R&D Systems

Product name

Mouse IL-21 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-21 in Western blots. Does not cross-react with recombinant human IL-21, recombinant mouse (rm) IL-15, or rmIL-12.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

Q9ES17


Isotype

IgG

Antibody clone number

149204

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells.

Pot C, Jin H, Awasthi A, Liu SM, Lai CY, Madan R, Sharpe AH, Karp CL, Miaw SC, Ho IC, Kuchroo VK
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 15;183(2):797-801

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-21 in Activated Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were untreated (light orange filled histogram) or activated for 24 hours with 50 ng/mL PMA and 500 ng/mL Ca²⁺ ionomycin (dark orange filled histogram) then stained with Mouse IL-21 Monoclonal Antibody (Catalog # MAB594) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂ Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.