Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [2]
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- Product number
- 13-7218-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-21 Monoclonal Antibody (eBio2B2-G20 (2B2-G20)), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody eBio2B2-G20 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15. Applications Reported: The eBio2B2-G20 antibody has been reported for use in ELISA detection of IL-21 analysis. Applications Tested: The eBio2B2-G20 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-21 (IL-21) in combination with the purified eBio3A3-N2 (Product # 14-7219-82) antibody for capture and recombinant human IL-21 (Product # 14-8219) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 60 pg/mL should be included in each ELISA plate. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- eBio2B2-G20 (2B2-G20)
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Synovial IL-21/TNF-producing CD4(+) T cells induce joint destruction in rheumatoid arthritis by inducing matrix metalloproteinase production by fibroblast-like synoviocytes.
Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function.
Lebre MC, Vieira PL, Tang MW, Aarrass S, Helder B, Newsom-Davis T, Tak PP, Screaton GR
Journal of leukocyte biology 2017 Mar;101(3):775-783
Journal of leukocyte biology 2017 Mar;101(3):775-783
Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function.
Parrish-Novak J, Dillon SR, Nelson A, Hammond A, Sprecher C, Gross JA, Johnston J, Madden K, Xu W, West J, Schrader S, Burkhead S, Heipel M, Brandt C, Kuijper JL, Kramer J, Conklin D, Presnell SR, Berry J, Shiota F, Bort S, Hambly K, Mudri S, Clegg C, Moore M, Grant FJ, Lofton-Day C, Gilbert T, Rayond F, Ching A, Yao L, Smith D, Webster P, Whitmore T, Maurer M, Kaushansky K, Holly RD, Foster D
Nature 2000 Nov 2;408(6808):57-63
Nature 2000 Nov 2;408(6808):57-63
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Standard curve of human IL-21 ELISA (Product # 88-7216).
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Standard curve of human IL-21 ELISA (Product # 88-7216).
- Conjugate
- Biotin