NBP1-76740

antibody from Novus Biologicals

Targeting: IL21 IL-21, Za11

ELISA

Immunocytochemistry

Antibody data

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Validation data

Product number: NBP1-76740 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-76740, RRID:AB_11021043
Product name: Rabbit Polyclonal IL-21 Antibody
Antibody type: Polyclonal
Description: Peptide affinity purified. Human IL-21 has 2 isoforms, including isoform 1 (162aa, 18.7kD) and isoform 2 (153aa, 17.6kD). Mouse IL-21 has one isoform (146aa, 16.8kD) and Rat IL-21 also has one isoform (146aa, 17.1kD). 2465 can detect both human isoforms and also detect mouse and rat. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 mg
Concentration: 1 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

IL21 protein structure - NBP1-76740 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IL-21 Antibody [NBP1-76740] - Analysis of IL21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with IL21 antibody using NBP1-76740 at 1 ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-21 recombinant protein per lane. Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human HL-60 Cell Lysate Loading: 15 ug of lysates per lane. Antibodies: IL-21 NBP1-76740 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. (A) Absence of blocking peptide (B) Presence of blocking peptide.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IL-21 Antibody [NBP1-76740] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: IL-21 NBP1-76740 (1 ug/mL), IL-21 from Company B (5 ug/mL), beta-actin (5 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: IL-21 NBP1-76740, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.