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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX632582 - Provider product page
- Provider
- GeneTex
- Product name
- Cofilin 1 antibody [GT217]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cofilin 1 antibody [GT217] detects Cofilin 1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Cofilin 1 protein from 293T whole cell extracts using 5 £gg of Cofilin 1 antibody [GT217] (GTX632582).Western blot analysis was performed using Cofilin 1 antibody [GT217] (GTX632582).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:250.
