PA5-27627

antibody from Invitrogen Antibodies

Targeting: CFL1 CFL

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-27627 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cofilin Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, NIH-3T3, PC-12, Rat2. Predicted reactivity: Mouse (98%), Rat (99%), Pig (99%), Sheep (99%), Rhesus Monkey (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CFL1 protein structure - PA5-27627 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cofilin 1 (non-muscle) in paraformaldehyde-fixed HeLa cells using a Cofilin 1 (non-muscle) polyclonal antibody (Product # PA5-27627) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Cofilin Cofilin in HeLa cells mock (left) and treated with 0.012 N NaOH/PBS for 1 min (right). Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cofilin Polyclonal Antibody (Product # PA5-27627) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Cofilin was performed using 70 confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cofilin Polyclonal Antibody (Product # PA5-27627) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Blue) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeleton, plasma membrane, nucleus and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60 magnification.