PA5-54107

antibody from Invitrogen Antibodies

Targeting: CBLB Cbl-b, RNF56

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54107

Provider

Invitrogen Antibodies

Product name

Cbl-b Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DNRLSRHIHH VESVPSRDPP MPLEAWCPRD VFGTNQLVGC RLLGEGSPKP GITASSNVNG RHSRVGSDPV LMRKHRRHDL PLEGAKVFSN GHLGSEEYDV PPRLSPPPPV TTLLPSIKCT GPLA Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 84%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cbl-b in control (vector only transfected HEK293T lysate) and CBLB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Cbl-b Polyclonal Antibody (Product # PA5-54107).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cbl-b Polyclonal Antibody (Product # PA5-54107) and a 110, 86 kDa band corresponding to Cbl-b was observed across cell models tested. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with Ionomycin (350 ng/ml) for 16 hours (Lane 2), BeWo (Lane 3), Reh (Lane 4), NIH:OVCAR-3 (Lane 5), Raji (Lane 6), HeLa (Lane 7), Mouse Cerebellum (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005). Induction of the target was observed in Jurkat cells upon treatment with Ionomycin. A non-specific band observed below the 60 kDa marker is marked with an asterisk.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Cbl-b in human cell line A-431 shows positivity in cytoplasm. Samples were probed using a Cbl-b Polyclonal Antibody (Product # PA5-54107).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Cbl-b in human cell line A-431 using a Cbl-b Polyclonal Antibody (Product # PA5-54107) shows localization to nucleoplasm and cytosol.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Cbl-b in human stomach tissue shows strong positivity in Parietal cells. Samples were probed using a Cbl-b Polyclonal Antibody (Product # PA5-54107).