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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [3]
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- Product number
- MA5-12888 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ErbB4 Monoclonal Antibody (H4.77.16 (Ab77))
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- MA5-12888 targets HER-4 in Western blot, flow cytometry and immunoprecipitation applications and shows reactivity with Human, mouse, and Rat samples.
- Antibody clone number
- H4.77.16 (Ab77)
- Concentration
- 0.2 mg/mL
Submitted references ErbB1-dependent signalling and vesicular trafficking in primary afferent nociceptors associated with hypersensitivity in neuropathic pain.
Neuregulin 3 promotes excitatory synapse formation on hippocampal interneurons.
Abnormal wiring of CCK(+) basket cells disrupts spatial information coding.
Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons.
Synergistic Chemoimmunotherapy of Epithelial Ovarian Cancer Using ErbB-Retargeted T Cells Combined with Carboplatin.
Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4).
Flexible targeting of ErbB dimers that drive tumorigenesis by using genetically engineered T cells.
Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons.
ErbB4 increases the proliferation potential of human lung cancer cells and its blockage can be used as a target for anti-cancer therapy.
Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum.
Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.
Regulation of ErbB4 phosphorylation and cleavage by a novel histidine acid phosphatase.
HER-2/neu overexpression increases the viable hypoxic cell population within solid tumors without causing changes in tumor vascularization.
Absence of endothelial cells, central necrosis, and fibrosis are associated with aggressive inflammatory breast cancer.
Anti-HER2 antibody enhances the growth inhibitory effect of anti-oestrogen on breast cancer cells expressing both oestrogen receptors and HER2.
Reversal of HER-2 over-expression renders human ovarian cancer cells highly resistant to taxol.
Reversal of HER-2 over-expression renders human ovarian cancer cells highly resistant to taxol.
Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo.
Dual effect of erbB-2 depletion on the regulation of DNA repair and cell cycle mechanisms in non-small cell lung cancer cells.
Mitchell R, Mikolajczak M, Kersten C, Fleetwood-Walker S
Neurobiology of disease 2020 Aug;142:104961
Neurobiology of disease 2020 Aug;142:104961
Neuregulin 3 promotes excitatory synapse formation on hippocampal interneurons.
Müller T, Braud S, Jüttner R, Voigt BC, Paulick K, Sheean ME, Klisch C, Gueneykaya D, Rathjen FG, Geiger JR, Poulet JF, Birchmeier C
The EMBO journal 2018 Sep 3;37(17)
The EMBO journal 2018 Sep 3;37(17)
Abnormal wiring of CCK(+) basket cells disrupts spatial information coding.
Del Pino I, Brotons-Mas JR, Marques-Smith A, Marighetto A, Frick A, Marín O, Rico B
Nature neuroscience 2017 Jun;20(6):784-792
Nature neuroscience 2017 Jun;20(6):784-792
Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons.
Lasiene J, Komine O, Fujimori-Tonou N, Powers B, Endo F, Watanabe S, Shijie J, Ravits J, Horner P, Misawa H, Yamanaka K
Acta neuropathologica communications 2016 Feb 18;4:15
Acta neuropathologica communications 2016 Feb 18;4:15
Synergistic Chemoimmunotherapy of Epithelial Ovarian Cancer Using ErbB-Retargeted T Cells Combined with Carboplatin.
Parente-Pereira AC, Whilding LM, Brewig N, van der Stegen SJ, Davies DM, Wilkie S, van Schalkwyk MC, Ghaem-Maghami S, Maher J
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 1;191(5):2437-45
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 1;191(5):2437-45
Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4).
Jay JI, Brunhoeber PS, Smith MH, Williams RR, Sugarman MC, Free HL, Tast DE
Histopathology 2013 Mar;62(4):563-77
Histopathology 2013 Mar;62(4):563-77
Flexible targeting of ErbB dimers that drive tumorigenesis by using genetically engineered T cells.
Davies DM, Foster J, Van Der Stegen SJ, Parente-Pereira AC, Chiapero-Stanke L, Delinassios GJ, Burbridge SE, Kao V, Liu Z, Bosshard-Carter L, Van Schalkwyk MC, Box C, Eccles SA, Mather SJ, Wilkie S, Maher J
Molecular medicine (Cambridge, Mass.) 2012 May 9;18:565-76
Molecular medicine (Cambridge, Mass.) 2012 May 9;18:565-76
Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons.
Longart M, Chatani-Hinze M, Gonzalez CM, Vullhorst D, Buonanno A
Brain research bulletin 2007 Jul 12;73(4-6):210-9
Brain research bulletin 2007 Jul 12;73(4-6):210-9
ErbB4 increases the proliferation potential of human lung cancer cells and its blockage can be used as a target for anti-cancer therapy.
Starr A, Greif J, Vexler A, Ashkenazy-Voghera M, Gladesh V, Rubin C, Kerber G, Marmor S, Lev-Ari S, Inbar M, Yarden Y, Ben-Yosef R
International journal of cancer 2006 Jul 15;119(2):269-74
International journal of cancer 2006 Jul 15;119(2):269-74
Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum.
Akayama Y, Takekida S, Ohara N, Tateiwa H, Chen W, Nakabayashi K, Maruo T
Human reproduction (Oxford, England) 2005 Oct;20(10):2708-14
Human reproduction (Oxford, England) 2005 Oct;20(10):2708-14
Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.
Horak E, Heitner T, Robinson MK, Simmons HH, Garrison J, Russeva M, Furmanova P, Lou J, Zhou Y, Yuan QA, Weiner LM, Adams GP, Marks JD
Cancer biotherapy & radiopharmaceuticals 2005 Dec;20(6):603-13
Cancer biotherapy & radiopharmaceuticals 2005 Dec;20(6):603-13
Regulation of ErbB4 phosphorylation and cleavage by a novel histidine acid phosphatase.
Fleisig H, El-Din El-Husseini A, Vincent SR
Neuroscience 2004;127(1):91-100
Neuroscience 2004;127(1):91-100
HER-2/neu overexpression increases the viable hypoxic cell population within solid tumors without causing changes in tumor vascularization.
Dragowska WH, Warburton C, Yapp DT, Minchinton AI, Hu Y, Waterhouse DN, Gelmon K, Skov K, Woo J, Masin D, Huxham LA, Kyle AH, Bally MB
Molecular cancer research : MCR 2004 Nov;2(11):606-19
Molecular cancer research : MCR 2004 Nov;2(11):606-19
Absence of endothelial cells, central necrosis, and fibrosis are associated with aggressive inflammatory breast cancer.
Shirakawa K, Tsuda H, Heike Y, Kato K, Asada R, Inomata M, Sasaki H, Kasumi F, Yoshimoto M, Iwanaga T, Konishi F, Terada M, Wakasugi H
Cancer research 2001 Jan 15;61(2):445-51
Cancer research 2001 Jan 15;61(2):445-51
Anti-HER2 antibody enhances the growth inhibitory effect of anti-oestrogen on breast cancer cells expressing both oestrogen receptors and HER2.
Kunisue H, Kurebayashi J, Otsuki T, Tang CK, Kurosumi M, Yamamoto S, Tanaka K, Doihara H, Shimizu N, Sonoo H
British journal of cancer 2000 Jan;82(1):46-51
British journal of cancer 2000 Jan;82(1):46-51
Reversal of HER-2 over-expression renders human ovarian cancer cells highly resistant to taxol.
Aigner A, Hsieh SS, Malerczyk C, Czubayko F
Toxicology 2000 Apr 3;144(1-3):221-8
Toxicology 2000 Apr 3;144(1-3):221-8
Reversal of HER-2 over-expression renders human ovarian cancer cells highly resistant to taxol.
Aigner A, Hsieh SS, Malerczyk C, Czubayko F
Toxicology 2000 Apr 3;144(1-3):221-8
Toxicology 2000 Apr 3;144(1-3):221-8
Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo.
Tang CK, Concepcion XZ, Milan M, Gong X, Montgomery E, Lippman ME
Cancer research 1999 Oct 15;59(20):5315-22
Cancer research 1999 Oct 15;59(20):5315-22
Dual effect of erbB-2 depletion on the regulation of DNA repair and cell cycle mechanisms in non-small cell lung cancer cells.
You XL, Yen L, Zeng-Rong N, Al Moustafa AE, Alaoui-Jamali MA
Oncogene 1998 Dec 17;17(24):3177-86
Oncogene 1998 Dec 17;17(24):3177-86
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HER-4 was done on 70% confluent log phase T47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HER-4 (H4.77.16 (Ab77)) Mouse Monoclonal Antibody (Product # MA5-12888) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic and membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HER-4 was done on T-47D cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with HER-4 Mouse Monoclonal Antibody (MA512888, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HER-4 in Neuro-2a cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a HER-4 monoclonal antibody (Product # MA5-12888) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HER-4 in T47D cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a HER-4 monoclonal antibody (Product # MA5-12888) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
