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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [1]
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- Product number
- MA5-13013 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ErbB4 Monoclonal Antibody (H4.72.8 (Ab72)), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-13013 targets HER-4 in WB and FACS applications and shows reactivity with Human samples.
- Conjugate
- Biotin
- Antibody clone number
- H4.72.8 (Ab72)
- Concentration
- 0.2 mg/mL
Submitted references Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4).
Neuregulin directly decreases voltage-gated sodium current in hippocampal ErbB4-expressing interneurons.
Slow down to stay alive: HER4 protects against cellular stress and confers chemoresistance in neuroblastoma.
Decorin, erythroblastic leukaemia viral oncogene homologue B4 and signal transducer and activator of transcription 3 regulation of semaphorin 3A in central nervous system scar tissue.
Heparin-binding epidermal growth factor-like growth factor is a potent neurotrophic factor for PC12 cells.
Populations of radial glial cells respond differently to reelin and neuregulin1 in a ferret model of cortical dysplasia.
Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth.
Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor.
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons.
ErbB4 increases the proliferation potential of human lung cancer cells and its blockage can be used as a target for anti-cancer therapy.
Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
Differential localization of ErbB receptor ensembles influences their signaling in hippocampal neurons.
Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.
An inhibitor of the EGF receptor family blocks myeloma cell growth factor activity of HB-EGF and potentiates dexamethasone or anti-IL-6 antibody-induced apoptosis.
Signaling pathways required for matrix metalloproteinase-9 induction by betacellulin in head-and-neck squamous carcinoma cells.
Neuregulin expression, function, and signaling in human ovarian cancer cells.
Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.
Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation.
Jay JI, Brunhoeber PS, Smith MH, Williams RR, Sugarman MC, Free HL, Tast DE
Histopathology 2013 Mar;62(4):563-77
Histopathology 2013 Mar;62(4):563-77
Neuregulin directly decreases voltage-gated sodium current in hippocampal ErbB4-expressing interneurons.
Janssen MJ, Leiva-Salcedo E, Buonanno A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Oct 3;32(40):13889-95
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Oct 3;32(40):13889-95
Slow down to stay alive: HER4 protects against cellular stress and confers chemoresistance in neuroblastoma.
Hua Y, Gorshkov K, Yang Y, Wang W, Zhang N, Hughes DP
Cancer 2012 Oct 15;118(20):5140-54
Cancer 2012 Oct 15;118(20):5140-54
Decorin, erythroblastic leukaemia viral oncogene homologue B4 and signal transducer and activator of transcription 3 regulation of semaphorin 3A in central nervous system scar tissue.
Minor KH, Bournat JC, Toscano N, Giger RJ, Davies SJ
Brain : a journal of neurology 2011 Apr;134(Pt 4):1140-55
Brain : a journal of neurology 2011 Apr;134(Pt 4):1140-55
Heparin-binding epidermal growth factor-like growth factor is a potent neurotrophic factor for PC12 cells.
Zhou Y, Besner GE
Neuro-Signals 2010;18(3):141-51
Neuro-Signals 2010;18(3):141-51
Populations of radial glial cells respond differently to reelin and neuregulin1 in a ferret model of cortical dysplasia.
Poluch S, Juliano SL
PloS one 2010 Oct 28;5(10):e13709
PloS one 2010 Oct 28;5(10):e13709
Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth.
Proietti CJ, Rosemblit C, Beguelin W, Rivas MA, Díaz Flaqué MC, Charreau EH, Schillaci R, Elizalde PV
Molecular and cellular biology 2009 Mar;29(5):1249-65
Molecular and cellular biology 2009 Mar;29(5):1249-65
Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor.
Leach RE, Kilburn BA, Petkova A, Romero R, Armant DR
American journal of obstetrics and gynecology 2008 Apr;198(4):471.e1-7; discussion 471.e7-8
American journal of obstetrics and gynecology 2008 Apr;198(4):471.e1-7; discussion 471.e7-8
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR
Biology of reproduction 2007 Jul;77(1):53-60
Biology of reproduction 2007 Jul;77(1):53-60
Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons.
Longart M, Chatani-Hinze M, Gonzalez CM, Vullhorst D, Buonanno A
Brain research bulletin 2007 Jul 12;73(4-6):210-9
Brain research bulletin 2007 Jul 12;73(4-6):210-9
ErbB4 increases the proliferation potential of human lung cancer cells and its blockage can be used as a target for anti-cancer therapy.
Starr A, Greif J, Vexler A, Ashkenazy-Voghera M, Gladesh V, Rubin C, Kerber G, Marmor S, Lev-Ari S, Inbar M, Yarden Y, Ben-Yosef R
International journal of cancer 2006 Jul 15;119(2):269-74
International journal of cancer 2006 Jul 15;119(2):269-74
Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE
Development (Cambridge, England) 2006 Feb;133(4):751-9
Development (Cambridge, England) 2006 Feb;133(4):751-9
Differential localization of ErbB receptor ensembles influences their signaling in hippocampal neurons.
Chen J, Tseng HC, Dichter MA, Zhang H, Greene MI
DNA and cell biology 2005 Sep;24(9):553-62
DNA and cell biology 2005 Sep;24(9):553-62
Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.
Horak E, Heitner T, Robinson MK, Simmons HH, Garrison J, Russeva M, Furmanova P, Lou J, Zhou Y, Yuan QA, Weiner LM, Adams GP, Marks JD
Cancer biotherapy & radiopharmaceuticals 2005 Dec;20(6):603-13
Cancer biotherapy & radiopharmaceuticals 2005 Dec;20(6):603-13
An inhibitor of the EGF receptor family blocks myeloma cell growth factor activity of HB-EGF and potentiates dexamethasone or anti-IL-6 antibody-induced apoptosis.
Mahtouk K, Jourdan M, De Vos J, Hertogh C, Fiol G, Jourdan E, Rossi JF, Klein B
Blood 2004 Mar 1;103(5):1829-37
Blood 2004 Mar 1;103(5):1829-37
Signaling pathways required for matrix metalloproteinase-9 induction by betacellulin in head-and-neck squamous carcinoma cells.
O-charoenrat P, Wongkajornsilp A, Rhys-Evans PH, Eccles SA
International journal of cancer 2004 Aug 20;111(2):174-83
International journal of cancer 2004 Aug 20;111(2):174-83
Neuregulin expression, function, and signaling in human ovarian cancer cells.
Gilmour LM, Macleod KG, McCaig A, Sewell JM, Gullick WJ, Smyth JF, Langdon SP
Clinical cancer research : an official journal of the American Association for Cancer Research 2002 Dec;8(12):3933-42
Clinical cancer research : an official journal of the American Association for Cancer Research 2002 Dec;8(12):3933-42
Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.
O-charoenrat P, Modjtahedi H, Rhys-Evans P, Court WJ, Box GM, Eccles SA
Cancer research 2000 Feb 15;60(4):1121-8
Cancer research 2000 Feb 15;60(4):1121-8
Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation.
Elenius K, Paul S, Allison G, Sun J, Klagsbrun M
The EMBO journal 1997 Mar 17;16(6):1268-78
The EMBO journal 1997 Mar 17;16(6):1268-78
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HER-4 was performed by loading 25 µg of mouse brain cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a HER-4 monoclonal antibody (Product # MA5-13016) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~180 kDa.
- Conjugate
- Biotin
