Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM00056PU-N - Provider product page
- Provider
- OriGene
- Product name
- ErbB 4 (ERBB4) (1230-1250) (incl. pos. control) mouse monoclonal antibody, clone 6C5, Purified
- Antibody type
- Monoclonal
- Description
- ErbB 4 (ERBB4) (1230-1250) (incl. pos. control) mouse monoclonal antibody, clone 6C5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ERBB4
- Isotype
- IgG
- Antibody clone number
- 6C5
- Vial size
- 100 µg
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection of endogenous erbB4: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB4-6C5 (0.5 ug/ ml) for 1h at 15-22°C and developed by ECL (exp. time: 30 sec). Lane 1: MDA-MB 231 Lane 2: MDA-MB 468 Lane 3: MCF-7 Lane 4: T47D Lane 5: SW480 Lane 6: SW620
- Validation comment
- WB
