13-9687-82

antibody from Invitrogen Antibodies
Targeting: ERBB4 ALS19, HER4
Provider product page for 13-9687-82
Western blot
Immunoprecipitation
Immunohistochemistry

Antibody data

Product number
13-9687-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
ErbB4 (Her4) Monoclonal Antibody (HFR1), Biotin, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody HFR1 recognizes human and mouse epidermal growth factor receptor 4 (ErbB4 or HER4). ErbB4 belongs to the family of receptor tyrosine kinases (RTK) of which HER1 (EGRF), HER2 (ErbB2), and HER3 (ErbB3) are members. RTKs function to affect a variety of cell processes including proliferation, differentiation, migration, survival and apoptosis and are often disregulated in cancer. The ErbB4 protein contains extracellular ligand binding domains, a transmembrane region, and a cytoplasmic tail containing the tyrosine kinase domain and the binding site for the HFR1 antibody. Upon ligand binding, ErbB4 homo- or hetero-dimerizes with other family members, becomes phosphorylated, and can be cleaved allowing translocation to the nucleus. The HRF1 antibody recognizes membrane-bound, cytoplasmic and nuclear ErbB4. Cytosolic ErbB4 expression was found to correlate with a positive prognosis in a subset of breast cancer patients, while nuclear ErbB4 expression was inversely correlated with tumor grade and aggressiveness of breast tumors. Applications Reported: This HFR1 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This HFR1 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Mouse
Conjugate
Biotin
Isotype
IgG
Antibody clone number
HFR1
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
ERBB4 protein structure - 13-9687-82 shown in red.