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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-33120 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33120, RRID:AB_10004034
- Product name
- Rabbit Polyclonal ErbB4/Her4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The significance of Her2 on androgen receptor protein stability in the transition of androgen requirement in prostate cancer cells.
Hsu FN, Yang MS, Lin E, Tseng CF, Lin H
American journal of physiology. Endocrinology and metabolism 2011 May;300(5):E902-8
American journal of physiology. Endocrinology and metabolism 2011 May;300(5):E902-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ErbB4/Her4 Antibody [NBP1-33120] - A: A431 5% SDS PAGE NBP1-33120 diluted at 1:5000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ErbB4/Her4 Antibody [NBP1-33120] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ErbB4/Her4 Antibody [NBP1-33120] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ErbB4/Her4 Antibody [NBP1-33120] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ErbB4/Her4 Antibody [NBP1-33120] - Immunoprecipitation of Her4/ ErbB4 protein from MCF-7 whole cell extract using 4.5 ug of Her4/ ErbB4 antibody [C1C3]. Western blot analysis was performed using Her4/ ErbB4 antibody [C1C3] and diluted at 1:500. EasyBlot HRP-conjugated anti rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ErbB4/Her4 Antibody [NBP1-33120] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
