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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29272 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ErbB4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, mouse brain, rat brain.
- Concentration
- 0.97 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ERBB4 using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an ERBB4 polyclonal antibody (Product # PA5-29272) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ERBB4 using 30 µg of A431 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an ERBB4 polyclonal antibody (Product # PA5-29272) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ErbB4 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ErbB4 Polyclonal Antibody (Product # PA5-29272) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ErbB4 Polyclonal Antibody (Product # PA5-29272). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Her4/ErbB4 antibody [C1C3] ErbB4 Polyclonal Antibody (Product # PA5-29272) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ErbB4 was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ErbB4 Polyclonal Antibody (Product # PA5-29272) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (Product # PA5-29272) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (Product # PA5-29272) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ERBB4 (v-erb-A) (Product # PA5-29272) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Her4 / ErbB4 protein from MCF-7 whole cell extract using 4.5 µg of ErbB4 Polyclonal Antibody (Product # PA5-29272). Western blot analysis was performed using ErbB4 Polyclonal Antibody (Product # PA5-29272) and diluted at 1:500. HRP-conjugated anti rabbit IgG antibody was used to detect the primary antibody.
