AM00057PU-N

antibody from Acris Antibodies GmbH

Targeting: ERBB4 ALS19, HER4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM00057PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00057PU-N, RRID:AB_977269

Product name

anti ERBB4 / HER4 pTyr1242 (incl. pos. Control)

Antibody type

Monoclonal

Antigen

Phosphopeptide conjugated to hemocyanin. Epitope: Phospho-Tyrosine 1242 NPDpYWNH

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4C6

Vial size

0.1 mg

Concentration

0.1 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

erbB4 activation Serum starved MDA-MB468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB4 - 4C6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF; lane 6: 2h EGF lane 7: 4h EGF; lane 8: 8h EGF