LS-C59301

antibody from LSBio

Targeting: GLUD1 GDH, GLUD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C59301

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C59301, RRID:AB_2110529

Product name

GLUD1/Glutamate Dehydrogenase Antibody LS-C59301

Antibody type

Polyclonal

Description

Delipidated and defibrinated

Reactivity

Bovine

Host

Rabbit

Storage

Store at 4°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.