R1086

antibody from Acris Antibodies GmbH

Targeting: GLUD1 GDH, GLUD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

R1086

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1086, RRID:AB_1001882

Product name

anti Glutamate dehydrogenase 1

Antibody type

Polyclonal

Antigen

A full length Glutamate Dehydrogenase protein isolated from Bovine Liver.

Reactivity

Bovine

Host

Rabbit

Vial size

2 ml

Concentration

85.0 mg/ml (by refractometry)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot analysis is shown using anti-Bovine Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 µm nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye(TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature. IRDye(TM) 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.