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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
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- Product number
- PA1-720 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDE6A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-720 detects phosphodiesterase 6 alpha (PDE6 alpha) from sheep retinal extract and bovine rod outer segment membranes as well as purified bovine PDE6. PA1-720 has been successfully used in Western blot and immunofluoresence procedures. By Western blot, this antibody detects an ~90 kDa protein representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts. The PA1-720 immunizing peptide corresponds to amino acid residues 1-16 from human PDE6 alpha. This sequence is completely conserved between human, mouse and bovine and 94% conserved in canine. PA1-720 immunizing peptide (Cat. # PEP-148) is available for use in neutralization and control experiments.
- Reactivity
- Human, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Regulation of rod photoreceptor function by farnesylated G-protein γ-subunits.
Essential role of the chaperonin CCT in rod outer segment biogenesis.
Leukemia inhibitory factor coordinates the down-regulation of the visual cycle in the retina and retinal-pigmented epithelium.
Effect of the ILE86TER mutation in the γ subunit of cGMP phosphodiesterase (PDE6) on rod photoreceptor signaling.
Function of the asparagine 74 residue of the inhibitory γ-subunit of retinal rod cGMP-phophodiesterase (PDE) in vivo.
Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.
In vitro interaction of tubulin with the photoreceptor cGMP phosphodiesterase gamma-subunit.
High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration.
Phototransduction in a transgenic mouse model of Nougaret night blindness.
The inhibitory gamma subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure.
GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.
Transducin activation state controls its light-dependent translocation in rod photoreceptors.
Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.
Enhancement of phototransduction g protein-effector interactions by phosphoinositides.
Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '.
Kolesnikov AV, Lobysheva E, Gnana-Prakasam JP, Kefalov VJ, Kisselev OG
PloS one 2022;17(8):e0272506
PloS one 2022;17(8):e0272506
Essential role of the chaperonin CCT in rod outer segment biogenesis.
Sinha S, Belcastro M, Datta P, Seo S, Sokolov M
Investigative ophthalmology & visual science 2014 May 22;55(6):3775-85
Investigative ophthalmology & visual science 2014 May 22;55(6):3775-85
Leukemia inhibitory factor coordinates the down-regulation of the visual cycle in the retina and retinal-pigmented epithelium.
Chucair-Elliott AJ, Elliott MH, Wang J, Moiseyev GP, Ma JX, Politi LE, Rotstein NP, Akira S, Uematsu S, Ash JD
The Journal of biological chemistry 2012 Jul 13;287(29):24092-102
The Journal of biological chemistry 2012 Jul 13;287(29):24092-102
Effect of the ILE86TER mutation in the γ subunit of cGMP phosphodiesterase (PDE6) on rod photoreceptor signaling.
Tsang SH, Woodruff ML, Lin CS, Jacobson BD, Naumann MC, Hsu CW, Davis RJ, Cilluffo MC, Chen J, Fain GL
Cellular signalling 2012 Jan;24(1):181-8
Cellular signalling 2012 Jan;24(1):181-8
Function of the asparagine 74 residue of the inhibitory γ-subunit of retinal rod cGMP-phophodiesterase (PDE) in vivo.
Tsang SH, Woodruff ML, Hsu CW, Naumann MC, Cilluffo M, Tosi J, Lin CS
Cellular signalling 2011 Oct;23(10):1584-9
Cellular signalling 2011 Oct;23(10):1584-9
Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.
Chen CK, Woodruff ML, Chen FS, Shim H, Cilluffo MC, Fain GL
The Journal of physiology 2010 Sep 1;588(Pt 17):3231-41
The Journal of physiology 2010 Sep 1;588(Pt 17):3231-41
In vitro interaction of tubulin with the photoreceptor cGMP phosphodiesterase gamma-subunit.
Chu UB, Song J, Mavlyutov TA, Guo LW
Neuroscience letters 2010 Oct 4;482(3):225-9
Neuroscience letters 2010 Oct 4;482(3):225-9
High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration.
Tanito M, Brush RS, Elliott MH, Wicker LD, Henry KR, Anderson RE
Journal of lipid research 2009 May;50(5):807-19
Journal of lipid research 2009 May;50(5):807-19
Phototransduction in a transgenic mouse model of Nougaret night blindness.
Moussaif M, Rubin WW, Kerov V, Reh R, Chen D, Lem J, Chen CK, Hurley JB, Burns ME, Artemyev NO
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jun 21;26(25):6863-72
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jun 21;26(25):6863-72
The inhibitory gamma subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure.
Guo LW, Muradov H, Hajipour AR, Sievert MK, Artemyev NO, Ruoho AE
The Journal of biological chemistry 2006 Jun 2;281(22):15412-22
The Journal of biological chemistry 2006 Jun 2;281(22):15412-22
GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.
Tsang SH, Woodruff ML, Chen CK, Yamashita CY, Cilluffo MC, Rao AL, Farber DB, Fain GL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 26;26(17):4472-80
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 26;26(17):4472-80
Transducin activation state controls its light-dependent translocation in rod photoreceptors.
Kerov V, Chen D, Moussaif M, Chen YJ, Chen CK, Artemyev NO
The Journal of biological chemistry 2005 Dec 9;280(49):41069-76
The Journal of biological chemistry 2005 Dec 9;280(49):41069-76
Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.
Guo LW, Grant JE, Hajipour AR, Muradov H, Arbabian M, Artemyev NO, Ruoho AE
The Journal of biological chemistry 2005 Apr 1;280(13):12585-92
The Journal of biological chemistry 2005 Apr 1;280(13):12585-92
Enhancement of phototransduction g protein-effector interactions by phosphoinositides.
He F, Mao M, Wensel TG
The Journal of biological chemistry 2004 Mar 5;279(10):8986-90
The Journal of biological chemistry 2004 Mar 5;279(10):8986-90
Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '.
Granovsky AE, Artemyev NO
The Journal of biological chemistry 2000 Dec 29;275(52):41258-62
The Journal of biological chemistry 2000 Dec 29;275(52):41258-62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of PDE6 alpha on sheep retinal extracts using Product # PA1-720.
