PA5-29761

antibody from Invitrogen Antibodies

Targeting: SARS1 SARS, SERS

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29761

Provider

Invitrogen Antibodies

Product name

Seryl-tRNA synthetase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, HeLa, HepG2. Predicted reactivity: Mouse (96%), Rat (94%), Zebrafish (81%), Xenopus laevis (84%), Chicken (87%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.51 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Seryl-tRNA synthetase using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Seryl-tRNA synthetase polyclonal antibody (Product # PA5-29761) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Seryl-tRNA synthetase antibody detects Seryl-tRNA synthetase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Seryl-tRNA synthetase antibody Seryl-tRNA synthetase Polyclonal Antibody (Product # PA5-29761) diluted by 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Seryl-tRNA synthetase (Product # PA5-29761) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Seryl-tRNA synthetase Polyclonal Antibody detects Seryl-tRNA synthetase protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Seryl-tRNA synthetase Polyclonal Antibody (Product # PA5-29761) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Seryl-tRNA synthetase Polyclonal Antibody detects Seryl-tRNA synthetase protein at cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. Seryl-tRNA synthetase Polyclonal Antibody (Product # PA5-29761) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Seryl-tRNA synthetase was performed in 293T whole cell extracts using 5 µg of Seryl-tRNA synthetase Polyclonal Antibody (Product # PA5-29761). Samples were transferred to a membrane and probed with Seryl-tRNA synthetase Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.