PA5-29761

antibody from Invitrogen Antibodies

Targeting: SARS1 SARS, SERS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29761

Provider

Invitrogen Antibodies

Product name

Seryl-tRNA synthetase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, HeLa, HepG2.

Concentration

0.51 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Seryl-tRNA synthetase using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Seryl-tRNA synthetase polyclonal antibody (Product # PA5-29761) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Seryl-tRNA synthetase antibody detects Seryl-tRNA synthetase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Seryl-tRNA synthetase antibody Seryl-tRNA synthetase Polyclonal Antibody (Product # PA5-29761) diluted by 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Seryl-tRNA synthetase (Product # PA5-29761) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Seryl-tRNA synthetase Polyclonal Antibody detects Seryl-tRNA synthetase protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Seryl-tRNA synthetase Polyclonal Antibody (Product # PA5-29761) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Seryl-tRNA synthetase Polyclonal Antibody detects Seryl-tRNA synthetase protein at cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. Seryl-tRNA synthetase Polyclonal Antibody (Product # PA5-29761) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Seryl-tRNA synthetase was performed in 293T whole cell extracts using 5 µg of Seryl-tRNA synthetase Polyclonal Antibody (Product # PA5-29761). Samples were transferred to a membrane and probed with Seryl-tRNA synthetase Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.