Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB300-538AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Galectin-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Galectin 3 (A3A12)
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
The epitope for this antibody has b
een mapped to the first 58 amino ac
ids of Galectin 3.- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Galectin-3 Antibody (A3A12) [Alexa Fluor 647] [NB300-538AF647] - An intracellular stain was performed on HT-29 cells with Galectin-3 (A3A12) antibody NB300-538AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.