- 39-5100 - Invitrogen Antibodies LGALS3 antibody

Xiao Y, Yang J, Fu Z, He D, Li N, Yuan J
Disease markers 2022;2022:1380560

Batista BS, Eng WS, Pilobello KT, Hendricks-Muñoz KD, Mahal LK
Journal of proteome research 2011 Oct 7;10(10):4624-33

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HT29 cell lysates using Zymed Ms anti-Galectin-3 (Product # 39-5100).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of Galectin3 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR855199_LV and CRISPR855204_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Galectin3 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHela Galectin3 KO (Lane 3) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Galectin 3 Monoclonal Antibody (ZG001) (Product # 39-5100, 1:2,000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:8,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to Galectin3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Galectin 3 Monoclonal Antibody (ZG001)(Product # 39-5100) and a ~30kDa band corresponding to Galectin 3 was observed across cell lines tested . Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), A549 (Lane 3), PC-3 (Lane 4), U-87 MG (Lane 5), A-431 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).a non specific band was observed around 62kDa and 85kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: The expression of galectin-3 protein determined by western blots and statistical difference in galectin-3 expression of WB results of corneal epithelial cells increased in the presence or absence of zymosan (a). Colocalization of CK12 and galectin-3 expression in corneal epithelial cells was determined by immunofluorescence analysis (b). There were more than 3 repetitive samples of each group. *: P < 0.05; **: P < 0.01; ***: P < 0.001; NS: no statistical difference.

39-5100