TA506398

antibody from OriGene

Targeting: LGALS3 GALIG, LGALS2, MAC-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506398

Provider

OriGene

Proper citation

OriGene Cat#TA506398, RRID:AB_2623755

Product name

LGALS3 mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)

Antibody type

Monoclonal

Description

LGALS3 mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)

Host

Mouse

Conjugate

Unconjugated

Epitope

LGALS3

Isotype

IgG

Antibody clone number

OTI1A4

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3 (RC208785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3 monoclonal antibody at 1:400 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-LGALS3 mouse monoclonal antibody (TA506398).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-LGALS3 mouse monoclonal antibody (TA506398) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGALS3(RC208785).

Validation comment

IF