14-5301-81

antibody from Invitrogen Antibodies

Targeting: LGALS3 GALIG, LGALS2, MAC-2

Provider product page for 14-5301-81

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [37]
Comments [0]
Validations
Western blot [3]
Immunocytochemistry [1]
Flow cytometry [1]
Other assay [30]

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Product number: 14-5301-81 - Provider product page
Provider: Invitrogen Antibodies
Product name: Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3). Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, immunohistology staining of paraffin embedded tissue sections, and ELISA. Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity: Human, Mouse
Host: Rat
Isotype: IgG
Antibody clone number: eBioM3/38 (M3/38)
Vial size: 50 µg
Concentration: 0.5 mg/mL
Storage: 4° C

LGALS3 protein structure - 14-5301-81 shown in red.

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Experimental details: Knockout of Galectin3 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR855199_LV and CRISPR855204_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Galectin3 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHela Galectin3 KO (Lane 3) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™ (Product # 14-5301-82, 0.5 µg/mL dilution) and F(ab)2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP (Product # PA1-29927, 1:6,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to Galectin3.

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Experimental details: Knockdown of Galectin 3 was achieved by transfecting MDA-MB-231 with Galectin 3 specific siRNAs (Silencer® select Product # S8149, S8148). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Galectin 3 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™ (Product # 14-5301-85, 0.5 µg/mL) and F(ab2-Rabbit anti-Rat IgG (H+L Secondary Antibody, HRP (Product # PA1-29927, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Galectin 3.^^13

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Experimental details: Western blot was performed using Anti-Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™(Product # 14-5301-85) and a ~30 kDa band corresponding to Galectin 3 was observed across cell lines tested . Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), A549 (Lane 3), PC-3 (Lane 4), U-87 MG (Lane 5), A-431 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with F(ab2-Rabbit anti-Rat IgG (H+L Secondary Antibody, HRP (Product # PA1-29927,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

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Supportive validation

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Supportive validation

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Experimental details: Figure 2 Gal3 positive CSC subset displays increased stemness characteristics. ( a ) ALDH activity was evaluated by flow cytometric analysis in Gal3 positive CSC (in red) and Gal3 negative CSC (in green). ALDH negative fractions are illustrated in gray. ( b ) Sphere formation ability (SFA) was determined. Gal3 positive CSC (red) accounted for significantly more spheres than Gal3 negative CSC (green). ( c ) TRAIL-resistance in Gal3 positive /Gal3 negative colon cancer CSC subsets was determined by APO-BRDU assay. Apoptotic cells are displayed as percentage of total cell count. ( d ) TRAIL-resistance in Gal3 positive /Gal3 negative CSC pancreatic cancer subsets. All experiments were performed in triplicate (see text for details). P

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Experimental details: Fig. 2 H&E staining, immunohistochemical staining for galectin-3, Iba-1 and GFAP, and apoptotic DNA fragmentation detected by TUNEL staining in a low-power field and a high-power field of the hippocampus at 96 h after ischemic insult. Many damaged CA2 neurons had lost their nuclear affinity for hematoxylin and appeared as an eosinophilic 'ghost neuron' in H&E staining. The localization of galectin-3-positive cells coincided with that of Iba-1-positive cells. There were no GFAP-positive cells in the CA2 damaged area. Some of the TUNEL-positive CA2 neurons were distinguishable among the CA1 neurons by the shape of positive stained nuclei. Scale bars in LPF and HPF of H&E staining are 100 and 50 mum, respectively. G3, galectin-3; GF, GFAP; HE, HE staining; HPF, high-power field; Iba, Iba-1; LPF, low-power field; TU, TUNEL staining.

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Experimental details: Figure 1 Surface Gal3 defines a subtype of epithelial CSC. ( a ) Colorectal cancer cells LiM6 and DLD1 were investigated by flow cytometry for cell surface markers CD24/CD44 (left). CD24+/CD44+-cells (in black) were then investigated for CD166/EpCAM-expression (middle) and CD24+/CD44+/CD166+/EpCAM+-positive cells (in black middle) for Gal3-expression (right panel), where ++++ Gal3 - in green refers to cells positive for CD24, CD44, CD166, and EpCAM, but negative for Gal3, and ++++ Gal3 + in red refers to cells positive for CD24, CD44, CD166, EpCAM, and Gal3. ( b ) Pancreatic cancer cell lines AsPC1 and L3.6pl were subjected to the same analysis as in ( a )

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Experimental details: FIGURE 2 Embryonic microglial cell population is poorly immunoreactive to the Mac-2/Galectin-3 antibody after single and double injection of poly (I:C). (A-F1) Coronal sections of embryonic brains, with cell nucleus staining in blue (DAPI) and microglial (CX3CR1-eGFP) cells in green. Immunohistochemical staining using a Mac-2 antibody (red) showed that at E17.5 almost no microglial cells in the cortex were immunoreactive for Mac-2 (A2) after injection with saline. At E11.5 (B2) and E17.5 (C2,E2) in the cortex and E17.5 hippocampal area (D2,F2) there was no increased percentage of microglial cells expressing the activation marker after poly (I:C) challenge compared to control. White square indicates the location of the cells in the tissue showed in the inset; * indicates a Mac-2 positive eGFP cell. Examples of one control brain area and poly (I:C) group only as they were not significantly different. Scale bar = 100 mum and for insets = 20 mum.

Supportive validation

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Experimental details: Figure 4 Silencing of Gal3 shifts CSC subset to a Gal3 negative CSC subset. ( a ) Western blot analysis for comparison of total Gal3 in LiM6TR or DLD1TR colon cancer cells and L3.6pl or AsPC1 pancreatic cancer cells after infection with lentiviral particles for control-shRNA ( c ) or Gal3-shRNA (G). Each pair of control and Gal3 knockdown cells were run side by side on the same gel. Comparison of Gal3 expression between cell lines has been previously published 10 ( b ) Flow cytometric analysis of colon cancer and pancreatic cancer cells after infection with lentiviral particles for control-shRNA (red trace) or Gal3-shRNA (green trace). Black trace is background staining. ( c ) ALDH activity was analyzed by flow cytometry. ALDEFLUOR activity in colon cancer and pancreatic cancer cells after infection with lentiviral particles for control-shRNA (blue trace) or Gal3-shRNA (green trace). Gray profile is background staining

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Experimental details: Figure 5 AdvSca1-SM cells adopt a myofibroblast, and only rarely macrophage, phenotype in response to vascular injury. Gli1 -Cre ERT -YFP mice were subjected to carotid arterial injury, and uninjured right and injured left carotids were harvested at 3 days, 7 days, and 3 weeks postinjury; fixed; and embedded in OCT. ( A ) Arterial sections were immunofluorescently stained for YFP (green), and in situ hybridization was used to detect periostin transcripts (red). Representative images from N = 3 at each time point. Note strong induction of periostin in AdvSca1-SM cells in response to injury. ( B and C ) Arterial sections were immunofluorescently stained for YFP (green) and CD68 ( B ; red) or MAC2 ( C ; red). Representative images from N = 3 per time point. Note that AdvSca1-SM cells do not coexpress either macrophage marker. Scale bars for panels A - C : 50 mum. M, arterial media; A, arterial adventitia; NI, neointima. Dashed lines indicate the external and internal elastic laminae. ( D ) Single-cell suspensions were isolated from uninjured and 7-day postinjured carotid arteries; cells were stained for SCA1, YFP, CD11c, MHCII, LY6G, CD11b, CD64, alphaSMA, and Aqua VI and analyzed by flow cytometry. Total macrophages (left graph) and YFP + AdvSca1-SM cell-derived macrophages (middle right) were quantified as percentage of all live cells. Median expression of alphaSMA (middle left) and CD64 (right) by the YFP + cell population was expressed as absolute value. Each point represent

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Experimental details: Fig. 4 Hydrogels evoke fibrotic responses at the material-tissue interface that can involve stromal or astroglial cells. a , b Detail images of stromal-associated markers, collagen-1a1 (Col1a1), fibronectin, laminin, and galectin-3 (Gal-3) and their relationship to non-neural CD13-positive cells at the tissue interface of DCH MO a and DCH K b at 1 week after injection (hydrogels, G). Colocalization of markers with CD13-positive cells is seen as white staining. c Quantification of total Col1a1 and Gal-3 staining at 1 week after hydrogel injection. *** P = 0.0003 and =0.0002 for DCH MO versus DCH K for Col1a1 and Gal-3 stromal markers, respectively, two-way ANOVA with Bonferroni. Graphs show mean +- s.e.m with individual data points showing n = 5 and 7 mice for DCH MO and DCH K , respectively. d Detail image showing that adjacent to DCH MO , Gal-3 colocalizes with a narrow band of GFAP-positive astrocytes that border the gel at 1 week. Gal-3 and GFAP colocalization is seen as white staining. e , f Detail images showing the temporal evolution of Gal-3 expression at the interface of DCH MO e and DCH K f at acute (48 h, 48 h), subacute (1 week, 1wk) and chronic (6 weeks, 6wk) timepoints after injection. Gal-3 and GFAP colocalization is seen as yellow staining. Gal-3 and CD13 colocalization is seen as white staining.

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Experimental details: Figure 2 Endothelial cell-specific KLF11 depletion aggravates Pcsk9 /AngII-induced AAA. The Pcsk9 /AngII-induced AAA model was performed on 8-week-old males with Klf11 ECKO ( n = 15) and their littermate Klf11 fl/fl ( n = 13) mice. ( A ) Schematics of Pcsk9 /AngII-induced AAA model. ( B ) Representative morphology of aortas from AngII-infused Klf11 fl/fl and Klf11 ECKO mice. ( C ) Incidence of AAA. ( D ) Maximal diameters of suprarenal abdominal aortas (SAAs) from AngII-infused Klf11 fl/fl ( n = 12) and Klf11 ECKO ( n = 14) mice. ( E ) Representative Verhoeff-Van Gieson (VVG) staining and quantification of elastin degradation in SAAs from AngII-infused Klf11 fl/fl ( n = 12) and Klf11 ECKO ( n = 14) mice. Scale bar: 50 mum. ( F ) Representative immunofluorescence staining and quantification of leukocyte (CD45 + ) and macrophage (Mac2 + ) infiltration in the aortic wall of SAAs from AngII-infused Klf11 fl/fl ( n = 12) and Klf11 ECKO ( n = 14) mice. Scale bar: 20 mum. ( G ) ELISA analysis of MCP-1 and IL-6 in the plasma from AngII-infused Klf11 fl/fl ( n = 12) and Klf11 ECKO ( n = 14) mice. ( H ) Representative TUNEL staining (green) and quantification of apoptotic cells in the media of SAAs from AngII-infused Klf11 fl/fl ( n = 12) and Klf11 ECKO ( n = 14) mice. Scale bar: 20 mum. ( I ) Representative DHE staining (red) and quantification of ROS production in the aortic wall of SAAs from AngII-infused Klf11 fl/fl ( n = 5) and Klf11 ECKO ( n = 6). Scale bar: 20 mum. Data are pres

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Experimental details: Figure 3 EC-selective overexpression of KLF11 attenuates Pcsk9 /AngII-induced AAA. The Pcsk9 /AngII-induced AAA model was performed on 10-week-old male EC-selective KLF11 transgenic mice ( Tie2-KLF11 -Tg, n = 12) and littermate control mice (WT, n = 13). ( A ) Representative morphology of aortas from AngII-infused WT and Tie2-KLF11 -Tg mice. ( B ) Incidence of AAA. ( C ) Maximal diameters of SAAs. ( D ) Representative VVG staining and quantification of elastin degradation in SAAs from WT and Tie2-KLF11 -Tg mice ( n = 10/group). Scale bar: 200 mum for whole aortic sections, 20 mum for magnified areas. ( E ) Representative immunofluorescence staining and quantification of leukocyte (CD45 + ) and macrophage (Mac2 + ) infiltration in the aortic wall of SAAs from WT and Tie2-KLF11 -Tg mice ( n = 10/group). Scale bar: 20 mum. ( F ) Representative TUNEL staining (green) and quantification of apoptotic cells in the media of SAAs from WT and Tie2-KLF11 -Tg mice ( n = 10/group). Scale bar: 20 mum. Data are presented as mean +- SEM. chi 2 test ( B ), Student''s 2-tailed t test ( C , E , and F ), Mann-Whitney U test ( D ).

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Experimental details: Fig. 1. Decreased macrophage accumulation in the adipose tissue of old Arg-II deficient (Arg-II -/- ) mice. a Representative confocal images of macrophage accumulation in the peri-uterus fat tissue of old WT and Arg-II -/- mice as assessed by immunofluorescence staining of paraffin-embedded sections with antibody against the macrophage marker Mac-2 (red), followed by counterstaining of the nuclei with DAPI (blue). Scale bar = 100 mum. b Quantification of the macrophage number per field is shown in the graph below (left panel), n = 4. c qRT-PCR analysis of IL-6 mRNA expression in the peri-uterus fat tissue, n = 8. d IL-6 protein level in the conditioned medium from peri-uterus fat tissues of mice measured by ELISA, n = 4. n indicates the number of animals of each experimental group. ** p < 0.01 between the indicated groups. Arg, arginase; WT, wild type; DAPI, 4'6-diamidino-2-phenyl-indole, dihydrochloride.

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Experimental details: Fig. 5. Localization of Arg-II in adipose tissues in aging. Confocal images of the localization of Arg-II in adipose tissues of old WT mice ( a ) and Arg-II -/- mice ( b ) as assessed by immunofluorescence staining of paraffin-embedded sections with antibodies against Mac-2 (red) and Arg-II (green), followed by counterstaining of the nuclei with DAPI (blue). Scale bar = 100 mum for lower amplification in the upper panel of a , b or 50 mum for zoomed images. Shown are representative images from 3 independent series of experiments. Arg, arginase; WT, wild type; DAPI, 4'6-diamidino-2-phenyl-indole, dihydrochloride.

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Experimental details: Fig. 1 Galectin-3 immunostaining in gerbil whole hippocampus including the CA2 sector at 60 h (upper) and 66 h (lower) after ischemic insult. In 60 h, weak immunoreactivity was recognized in CA2 (surrounded by arrowheads). In 66 h, prominent immunostaining was observed in CA2 (surrounded by arrowheads). Ependymal cells lining the lateral ventricles are strongly positive for galectin-3 as an internal positive control. The scale bar in the upper photograph is 500 mum.

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Experimental details: Fig 1 Time-course of representative microphotographs of H&E staining, Azan staining for fibrotic lesions, and immunohistochemistry for galectin-3 and Iba1 in heart tissues at 0, 12, 24, 48, 96 hours, 7 and 10 days after intraperitoneal inoculation of EMCV. For both H&E and Azan staining, notable changes was not observed until 48 hours after EMCV inoculation. Infiltration of inflammatory cells and fibrosis was observed 96 hours after inoculation. Inflammation and fibrosis peaked at 7 days after inoculation. At 0, 12 and 24 hours, there were no galectin-3 and Iba1-positive cell in heart tissues. At 48 hours after EMCV inoculation, interstitial infiltration of a few cells was detected by both galectin-3 and Iba1 immunohistochemistry, and this infiltration was greater at 96 hours. It peaked on day 7 and was observed until day 10. Scale bar = 100 mum.

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Experimental details: 10.1371/journal.pone.0210971.g002 Fig 2 Immunofluorescence images confirming the co-localization of galectin-3 and Iba1 immunoreactivity in activated macrophages/histiocytes in myocarditis lesion 96 hours after EMCV inoculation. Galectin-3 and Iba1 were expressed in the same cells of merged photograph, indicating that infiltrating galectin-3 positive cells are macrophages. Scale bar = 100 mum.

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Experimental details: Fig 3 Quantification and association between the galectin-3 positive cell number and the degree of fibrosis. A, The number of galectin-3 positive cells (determined by immunohistochemistry) showing myocardial infiltration was counted at each time point. B, The degree of myocardial fibrosis (determined by of Azan staining) was quantified as percentage of fibrotic area to total myocardial tissue at each time point. C, Association between galectin-3 positive cell number and degree of fibrosis. The galectin-3 positive cell numbers were positively correlated with the degree of myocardial fibrosis. *Statistically different from each group (p < 0.05), determined by ANOVA.

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Experimental details: Figure 2 Macrophage NCOR1 (nuclear receptor corepressor 1) deficiency inhibits proliferation of cardiac macrophages after myocardial infarction. A , Representative immunofluorescence staining of MAC2 and BrdU in peri-infarct region of hearts from littermate control and macrophage Ncor1 knockout mice 7 days after myocardial infarction. The arrows point to MAC2/BrdU double-positive cells. Scale bar: 200 um. B , Quantification of MAC2-positve cells and MAC2/BrdU double-positive cells. n=3:3. C , Representative flow cytometry analysis of macrophages in whole hearts from littermate control and macrophage Ncor1 knockout mice 7 days after myocardial infarction. D , Quantification of CD11B + F4/80 + macrophages. n=5:4. E , Representative flow cytometry analysis of KI67 + macrophages in whole hearts from littermate control and macrophage Ncor1 knockout mice 7 days after myocardial infarction. F , Quantification of KI67 + macrophages. n=5:4. Values are expressed as mean+-SEM. BrdU indicates bromodeoxyuridine; KI67, nuclear proliferation antigen; LC, littermate control; MAC2, galectin3; MNKO, macrophage Ncor1 knockout; and SSC, side scatter. * P

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Experimental details: Figure 3 Macrophage NCOR1 (nuclear receptor corepressor 1) deficiency inhibits arterial injury-induced neointimal hyperplasia. A , Representative hematoxylin and eosin staining of cross section of femoral arteries from littermate control and macrophage Ncor1 knockout mice 28 days after wire injury. B , Quantification of intima area and intima to media ratio. n=7:7. C , Representative immunofluorescence staining of alpha-smooth muscle actin in injured arteries. D , Quantification of smooth muscle actin -positive cells. n=5:4. E , Quantitative reverse transcription polymerase chain reaction analysis of inflammatory genes in femoral arteries from littermate control and macrophage Ncor1 knockout mice 7 days after wire injury. Femoral arteries isolated from 3 to 4 mice were mixed together as 1 technical sample, and 3 technical samples were used in each group. F , Representative immunofluorescence staining of MAC2 and BrdU in injured femoral arteries from littermate control and macrophage Ncor1 knockout mice 21 days after wire injury. The arrows point to MAC2/BrdU double-positive cells. G , Quantification of MAC2-positive cells and MAC2/BrdU double-positive cells. n=5:5. All scale bars: 200 um. Values are expressed as mean+-SEM. Ccl2 indicates C-C motif chemokine ligand 2; Cxcl1 , C-X-C motif chemokine ligand 1; Il-1b , interleukin 1 beta; Il-6 , interleukin 6; LC, littermate control; MNKO, macrophage Ncor1 knockout; Nos2 , nitric oxide synthase 2; ns, not significant; and Tnfa , t

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Experimental details: Fig. 2 miNano targets CCs and macrophages in atherosclerotic plaques in vivo. Apoe -deficient mice fed on a western diet or C57BL/6J mice fed on a standard laboratory diet for 12 weeks were given one dose of vehicle or 250 mg/kg DiD-miNano via tail vein ( n = 4). 24 h later, aortas were collected for ( a ) imaging DiD by IVIS and lipids by ORO stain; Aortic sinus was sectioned for ( b and c ) visualizing CCs under polarized light and ( d and e ) staining macrophages by galectin 3 antibody. ( b ) Representative merged images of DAPI-stained aortic sinus slides under a fluorescent microscope. ( c ) Magnified images from the yellow box in ( b ) under separated channels. ( d ) Representative merged images of DAPI- and galectin 3-stained aortic sinus slides under a confocal microscope. ( e ) Magnified images from the yellow box in ( d ) under separated channels. ( b - e ) DiD-miNano was detected by Cy5 fluorescent signal. Scale bars: ( b ) 200 mum, ( c-e ) 100 mum. Fig 2

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Experimental details: Fig. 3 miNano inhibits atherosclerosis in Ldlr -deficient mice. ( a ) Schematic diagram of treatment. 7, 8-week-old male and female Ldlr -deficient mice were challenged with a western diet for 12 weeks to establish atherosclerosis. Mice were then fed on a normal laboratory diet and randomized to either be sacrificed as a baseline or treated by vehicle, 250 mg/kg miNano, or 400 mg/kg HPBCD twice a week via tail vein injection for 6 weeks. Mice were sacrificed 48 h after the last injection at the end of the 18th week. Aortas were isolated for ORO staining, and aortic sinus was sectioned for galectin 3 staining to detect macrophages and Masson's trichrome staining to detect necrotic core and collagen content ( n = 6-10). ( b ) Representative images and quantification of plaque area in ( c ) male and ( d ) female aortas stained by ORO. ( e ) Representative images and quantification of ( f, g ) CCs and ( h, i ) galectin 3-positive area in aortic sinus sections from male and female mice. Quantification of ( j, k ) necrotic core area and ( l, m ) collagen content by Masson's trichrome staining in aortic sinus sections from male and female mice. Data are mean +- SD. Statistical difference was determined by ordinary one-way ANOVA and followed by Sidak's multiple comparisons test or Tukey's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bars: the left two panels of ( e ) 200 mum, the right two panels of ( e ) 100 mum. Abbreviation: PL, polarized light. Fig 3

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Experimental details: Figure 4. T-cell mineralocorticoid receptor (MR) deficiency attenuates angiotensin II (Ang II)-induced vascular damage. A , Representative hematoxylin and eosin staining of aortic sections. B , Quantification of aortic wall thickness (n=6-8). C , Representative picrosirius red staining of aortic sections. Fibrotic tissues stained red. D , Quantification of aortic fibrotic areas (n=8). E , Acetylcholine dose-response curves of mesenteric arteries (n=6-7). F , Representative immunofluorescence staining of Mac2 in aortic sections. G , Quantification of Mac2-positive cells (n=5-6). H , QRT-polymerase chain reaction (PCR) analysis of CD68 gene expression in aortas (n=6-8). I , QRT-PCR analysis of RANTES (regulated upon activation normal T-cell expressed and secreted) and monocyte chemotactic protein (MCP)-1 gene expression in aortas (n=6-8). J , Representative high-performance liquid chromatography tracings of 2-hydroxyethidium (2-HE &plus ) in aortic samples. K , Quantification of relative 2-HE &plus content (n=4-5). Mice were infused with vehicle or AngII for 4 wk. All scale bars, 50 &mgrm. &ast P

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Experimental details: Figure 1 ESCRT-III subunits are enriched in damage-prone cross-presenting CD8 + cDC1s (A) Purified splenic cDCs were incubated with beta-lactamase for various times before CCF4 loading, antibody staining, and analysis by flow cytometry (n = 3 experiments performed with technical duplicates or triplicates, two-way ANOVA, mean +- SEM, exact p values are indicated). The zero time point corresponds to incubation for 5 h without beta-lactamase at 37degC. (B) Splenic CD8 + cDC1s or CD11b + cDC2s were purified from FLT3L-treated mice and pulsed for 30 min with OVA-biotin. After a 2 h chase, cells were subjected to cell fractionation, and western blotting was then performed on cytosolic extracts obtained by ultracentrifugation. Blots representative of 7 experiments are shown. (C) Purified splenic cDCs were purified and treated for 30 min with 0.3 mM LLOME, endotoxin-containing (sOVA), or endotoxin-free (sEF-OVA) OVA for 4 h before fixation and galectin-3 intracellular staining. Images shown were acquired with a confocal microscope equipped with a 100x objective (scale bar, 5 mum). Frequencies (mean) in the right panel display the results of 3 (DMSO, LLOME, and sOVA) or 2 (sEF-OVA) independent experiments. Arrowheads indicate examples of galectin-3 focus location. (D) Intracellular compartments of CD8 + cDC1s or CD11b + cDC2s purified from B16-FLT3L-injected mice were isolated by subcellular fractionation and analyzed by mass spectrometry. Purity control (top panel), ESCRT-III compone

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Experimental details: Figure 4 ESCRT-III-silenced cDCs display increased antigen export to the cytosol (A) Timeline for the beta-lactamase assay in (B) and (C). (B) Representative plots after 3 h of incubation with or without beta-lactamase (gated on live cells). (C) Quantification of 4 independent experiments (two-way ANOVA, mean +- SEM, exact p values are indicated). The zero time point corresponds to incubation for 5 h without beta-lactamase at 37degC. (D-F) ESCRT-III-deficient or control MutuDCs were pulsed with tetramethylrhodamine-labeled dextrans (3K [D], 10K [E], or 70K [F]) before extensive washes and subsequent chase. Representative images acquired with a confocal microscope equipped with a 40x objective are shown (scale bar, 5 mum). Quantification of the median of cytosolic fluorescence and of the ratio of dextran + vesicle area on total cell area are shown (Student's t test, mean, exact p values are indicated). Each dot represents one cell. One experiment representative of 2 independent experiments is shown. Arrowheads indicate cells showing cytosolic dextran fluorescence. Because the images shown in (D) originate from the same experiment as the one presented in Figure S3 F, the control shRNA image and values are identical (and copied) in both figures. (G) qRT-PCR expression analysis of endogenous Chmp4b (left panel) and shRNA-resistant Chmp4b (right panel) in MutuDCs expressing (resistant CHMP4b) or not expressing (empty vector) shRNA-resistant CHMP4b and the different shRNA. n = 3 (u

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Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 A, Galectin-3 immunostaining in liver and kidney medulla and cortex. Representative images are shown. C, healthy control; DM (ETOH), type 2 diabetic animal; DM (8PN), diabetic animal supplemented with 8PN; DM (XN), diabetic animal supplemented with XN. Magnification 100x. B, Western blot for galectin-3 expression in liver and kidney. beta-Actin expression was used as loading control. A representative blot is shown. Graphs represent band intensity mean values. * rho < 0.05 versus control; ** rho < 0.001 versus control; *** rho < 0.0001 versus control. DM = diabetes mellitus; 8PN = 8-prenylnaringenin; XN = xanthohumol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Expression of galectin 3 is increased in mice on a high-fat diet. Immunohistochemical expression of galectin 3 + cells in islets. The number of galectin 3 immunopositive cells was significantly higher in TG mice compared to WT mice on standard diet. On HFD, the number of galectin 3 immunopositive cells in islets increased in both groups and was significantly higher in TG mice compared to WT (A) . Levels of galectin 3 in sera. By week 16 of HFD, levels of galectin 3 in sera were also significantly higher in TG mice compared to WT mice (B) . An analysis was performed by light microscope using a 40X magnifying lens. Results are presented as a mean count of positive cells per field on 10 non-overlapping fields/section. Data from two experiments with 10-15 mice per group are shown as mean +- SEM; by Mann-Whitney U -test and independent-sample Student's t -test; * p < 0.05.