14-5301-82

antibody from Invitrogen Antibodies
Targeting: LGALS3 GALIG, LGALS2, MAC-2
Provider product page for 14-5301-82
Western blot
ELISA
Immunocytochemistry
Immunoprecipitation
Immunohistochemistry
Flow cytometry
Other assay

Antibody data

Product number
14-5301-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3). Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, immunohistology staining of paraffin embedded tissue sections, and ELISA. Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Rat
Isotype
IgG
Antibody clone number
eBioM3/38 (M3/38)
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
LGALS3 protein structure - 14-5301-82 shown in red.