Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-5301-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3). Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- eBioM3/38 (M3/38)
- Vial size
- 50 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.
Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.
Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.
Ilmer M, Mazurek N, Byrd JC, Ramirez K, Hafley M, Alt E, Vykoukal J, Bresalier RS
Cell death & disease 2016 Aug 11;7(8):e2337
Cell death & disease 2016 Aug 11;7(8):e2337
Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.
Smolders S, Smolders SM, Swinnen N, Gärtner A, Rigo JM, Legendre P, Brône B
Frontiers in cellular neuroscience 2015;9:301
Frontiers in cellular neuroscience 2015;9:301
Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.
Madireddi S, Eun SY, Lee SW, Nemčovičová I, Mehta AK, Zajonc DM, Nishi N, Niki T, Hirashima M, Croft M
The Journal of experimental medicine 2014 Jun 30;211(7):1433-48
The Journal of experimental medicine 2014 Jun 30;211(7):1433-48
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of C57BL/6 bone marrow cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.03 µg of Anti-Human/Mouse Galectin-3 PE (right). Total viable cells were used for analysis.
- Conjugate
- Yellow dye