Explore
Validate
Learn
Western blot
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 126702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#126702, RRID:AB_1134256
- Product name
- Purified anti-mouse/human Mac-2 (Galectin-3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant (partial), amino acids 151-251
- Description
- Galectin-3 (galactose-specific lectin 3) is a 30-32 kD glycoprotein, also know as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-galectin-3 antibody (clone Gal397). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
