60207-1-Ig

antibody from Proteintech Group

Targeting: LGALS3 GALIG, LGALS2, MAC-2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

60207-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#60207-1-Ig, RRID:AB_10951109

Product name

Galectin-3 antibody

Antibody type

Monoclonal

Description

KD/KO validated Galectin-3 antibody (Cat. #60207-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

1C1B2

Vial size

20ul, 150ul

References

Submitted references

VCP Inhibition Augments NLRP3 Inflammasome Activation.

Sharma A, Dhavale DD, Kotzbauer PT, Weihl CC
Inflammation 2024 Oct;47(5):1868-1883

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Disulfiram Improves Fat Graft Retention by Modulating Macrophage Polarization With Inhibition of NLRP3 Inflammasome-Mediated Pyroptosis.

Chen X, Chen W, Xu H, Tian Y, Wang X, Chen X, Li J, Luo S, Hao L
Aesthetic surgery journal 2024 Jun 14;44(7):NP501-NP518

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TFEB ameliorates DEHP-induced neurotoxicity by activating GAL3/TRIM16 axis dependent lysophagy and alleviating lysosomal dysfunction.

Xing H, Xu P, Ma Y, Li T, Zhang Y, Ding X, Liu L, Keerman M, Niu Q
Environmental toxicology 2024 Jul;39(7):3779-3789

---

Surplus fatty acid synthesis increases oxidative stress in adipocytes and lnduces lipodystrophy.

Weng L, Tang WS, Wang X, Gong Y, Liu C, Hong NN, Tao Y, Li KZ, Liu SN, Jiang W, Li Y, Yao K, Chen L, Huang H, Zhao YZ, Hu ZP, Lu Y, Ye H, Du X, Zhou H, Li P, Zhao TJ
Nature communications 2024 Jan 2;15(1):133

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Zinc ions regulate mitochondrial quality control in neurons under oxidative stress and reduce PANoptosis in spinal cord injury models via the Lgals3-Bax pathway.

Bai M, Cui Y, Sang Z, Gao S, Zhao H, Mei X
Free radical biology & medicine 2024 Aug 20;221:169-180

---

Lysosomal destabilization: A missing link between pathological calcification and osteoarthritis.

Ye T, Wang C, Yan J, Qin Z, Qin W, Ma Y, Wan Q, Lu W, Zhang M, Tay FR, Jiao K, Niu L
Bioactive materials 2024 Apr;34:37-50

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SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis.

Xia Q, Zheng H, Li Y, Xu W, Wu C, Xu J, Li S, Zhang L, Dong L
Autophagy 2024 Apr;20(4):735-751

---

Transmission of NLRP3-IL-1β Signals in Cerebral Ischemia and Reperfusion Injury: from Microglia to Adjacent Neuron and Endothelial Cells via IL-1β/IL-1R1/TRAF6.

Pan J, Peng J, Li X, Wang H, Rong X, Peng Y
Molecular neurobiology 2023 May;60(5):2749-2766

---

Choline-deficient Diet-induced NAFLD Animal Model Recaptures Core Human Pathophysiology With Similar Gene Co-expression Networks.

Ishigure T, Sasase T, Tohma M, Uno K, Toriniwa Y, Saito T, Saigo Y, Edamura K, Miyajima K, Ohta T
In vivo (Athens, Greece) 2023 Jul-Aug;37(4):1517-1531

---

Mechano-dependent sorbitol accumulation supports biomolecular condensate.

Torrino S, Oldham WM, Tejedor AR, Burgos IS, Rachedi N, Fraissard K, Chauvet C, Sbai C, O'Hara BP, Abélanet S, Brau F, Clavel S, Collepardo-Guevara R, Espinosa JR, Ben-Sahra I, Bertero T
bioRxiv : the preprint server for biology 2023 Jul 25;

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Involvement of Galectin-3 in neurocognitive impairment in obstructive sleep apnea via regulating inflammation and oxidative stress through NLRP3.

Zong D, Liu X, Shen C, Liu T, Ouyang R
Sleep medicine 2023 Jan;101:1-10

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Leucine-rich repeat kinase 2 (LRRK2) inhibition upregulates microtubule-associated protein 1B to ameliorate lysosomal dysfunction and parkinsonism.

Chen K, Tang F, Du B, Yue ZZ, Jiao LL, Ding XL, Tuo QZ, Meng J, He SY, Dai L, Lei P, Wei XW
MedComm 2023 Dec;4(6):e429

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Catalpol Prevents Glomerular Angiogenesis Induced by Advanced Glycation End Products via Inhibiting Galectin-3.

Sun WX, Gao YY, Cao Y, Lu JF, Lv GH, Xu HQ
Current medical science 2023 Aug;43(4):668-678

---

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis.

Liu X, Tan S, Liu H, Jiang J, Wang X, Li L, Wu B
Hepatology (Baltimore, Md.) 2023 Apr 1;77(4):1181-1197

---

Modified Citrus Pectin Alleviates Cerebral Ischemia/Reperfusion Injury by Inhibiting NLRP3 Inflammasome Activation via TLR4/NF-ĸB Signaling Pathway in Microglia.

Cui Y, Zhang NN, Wang D, Meng WH, Chen HS
Journal of inflammation research 2022;15:3369-3385

---

Single-cell RNA sequencing reveals a novel inhibitory effect of ApoA4 on NAFL mediated by liver-specific subsets of myeloid cells.

Liu XH, Zhou JT, Yan CX, Cheng C, Fan JN, Xu J, Zheng Q, Bai Q, Li Z, Li S, Li X
Frontiers in immunology 2022;13:1038401

---

Transcriptomic Analysis Reveals that Activating Transcription Factor 3/c-Jun/Lgals3 Axis Is Associated with Central Diabetes Insipidus after Hypothalamic Injury.

Zhou M, Ou Y, Wu G, Li K, Peng J, Wang X, Che M, Gong H, Niu P, Liu Y, Feng Z, Qi S
Neuroendocrinology 2022;112(9):874-893

---

CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer.

Hong CL, Yu IS, Pai CH, Chen JS, Hsieh MS, Wu HL, Lin SW, Huang HP
Cancer research 2022 Oct 17;82(20):3734-3750

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Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression.

Pang Y, Maxwell E, Sindrewicz-Goral P, Shapanis A, Li S, Morgan M, Yu LG
Biomolecules 2022 Oct 10;12(10)

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Thiamine pretreatment improves endotoxemia-related liver injury and cholestatic complications by regulating galactose metabolism and inhibiting macrophage activation.

Li T, Bai J, Du Y, Tan P, Zheng T, Chen Y, Cheng Y, Cai T, Huang M, Fu W, Wen J
International immunopharmacology 2022 Jul;108:108892

---

17β-Estradiol Attenuates LPS-Induced Macrophage Inflammation In Vitro and Sepsis-Induced Vascular Inflammation In Vivo by Upregulating miR-29a-5p Expression.

Zhang ML, Chen H, Yang Z, Zhang MN, Wang X, Zhao K, Li X, Xiu N, Tong F, Wang YX
Mediators of inflammation 2021;2021:9921897

---

Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy.

Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
eLife 2021 Sep 29;10

---

Galectin‑3 facilitates the proliferation and migration of nasopharyngeal carcinoma cells via activation of the ERK1/2 and Akt signaling pathways, and is positively correlated with the inflammatory state of nasopharyngeal carcinoma.

Li M, Chen YB, Liu F, Qu JQ, Ren LC, Chai J, Tang CE
Molecular medicine reports 2021 May;23(5)

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Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis.

Meng X, Wang J, Zhou J, Tian Q, Qie B, Zhou G, Duan W, Zhu Y
Acta biomaterialia 2021 Jun;127:266-275

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Pro64His (rs4644) Polymorphism Within Galectin-3 Is a Risk Factor of Differentiated Thyroid Carcinoma and Affects the Transcriptome of Thyrocytes Engineered via CRISPR/Cas9 System.

Corrado A, Aceto R, Silvestri R, Dell'Anno I, Ricci B, Miglietta S, Romei C, Giovannoni R, Poliseno L, Evangelista M, Vitiello M, Cipollini M, Garritano S, Giusti L, Zallocco L, Elisei R, Landi S, Gemignani F
Thyroid : official journal of the American Thyroid Association 2021 Jul;31(7):1056-1066

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Yin-xing-tong-mai decoction attenuates atherosclerosis via activating PPARγ-LXRα-ABCA1/ABCG1 pathway.

Zheng S, Huang H, Li Y, Wang Y, Zheng Y, Liang J, Zhang S, Liu M, Fang Z
Pharmacological research 2021 Jul;169:105639

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Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.

Zou L, Zhang X, Xiong M, Meng L, Tian Y, Pan L, Yuan X, Chen G, Wang Z, Bu L, Yao Z, Zhang Z, Ye K, Zhang Z
Neurobiology of disease 2021 Jul;154:105326

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Galectin-3 Contributes to the Inhibitory Effect of lα,25-(OH)(2)D(3) on Osteoclastogenesis.

Gu J, Zhang X, Zhang C, Li Y, Bian J, Liu X, Yuan Y, Zou H, Tong X, Liu Z
International journal of molecular sciences 2021 Dec 11;22(24)

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Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Yang GS, Zheng B, Qin Y, Zhou J, Yang Z, Zhang XH, Zhao HY, Yang HJ, Wen JK
Theranostics 2020;10(17):7787-7811

---

Fine particulate matter inhibits phagocytosis of macrophages by disturbing autophagy.

Li Y, Yong YL, Yang M, Wang W, Qu X, Dang X, Shang D, Shao Y, Liu J, Chang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Dec;34(12):16716-16735

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Galectin-3 expression and secretion by tumor-associated macrophages in hypoxia promotes breast cancer progression.

Wang L, Li YS, Yu LG, Zhang XK, Zhao L, Gong FL, Yang XX, Guo XL
Biochemical pharmacology 2020 Aug;178:114113

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Leukemia cells apoptosis by a newly discovered heterogeneous polysaccharide from Angelica sinensis (Oliv.) Diels.

Liu W, Xiao K, Ren L, Sui Y, Chen J, Zhang T, Li XQ, Cao W
Carbohydrate polymers 2020 Aug 1;241:116279

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Gasdermin D Drives the Nonexosomal Secretion of Galectin-3, an Insulin Signal Antagonist.

Chen Y, Wang H, Shen J, Deng R, Yao X, Guo Q, Lu A, Sun B, Zhang Y, Meng G
Journal of immunology (Baltimore, Md. : 1950) 2019 Nov 15;203(10):2712-2723

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Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension.

Luo H, Liu B, Zhao L, He J, Li T, Zha L, Li X, Qi Q, Liu Y, Yu Z
Journal of the American Society of Hypertension : JASH 2017 Oct;11(10):673-683.e3

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Hypermethylation of IFN-γ in oral cancer tissues.

Tian S, Jiang C, Liu X, Xu S, Zhang Z, Chen H, Zhang Y, Liu Y, Ma D
Clinical oral investigations 2017 Nov;21(8):2535-2542

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Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Ma D, Zheng B, Suzuki T, Zhang R, Jiang C, Bai D, Yin W, Yang Z, Zhang X, Hou L, Zhan H, Wen JK
Circulation research 2017 Mar 3;120(5):799-815

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Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Tummala KS, Brandt M, Teijeiro A, Graña O, Schwabe RF, Perna C, Djouder N
Cell reports 2017 Apr 18;19(3):584-600

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Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages.

Jiang W, Zheng B, Zhang XH, Yue LY, Liu C, Ma D, Yang Z, Wen JK
American journal of translational research 2016;8(11):4778-4790

---

miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells.

Yang Z, Zheng B, Zhang Y, He M, Zhang XH, Ma D, Zhang RN, Wu XL, Wen JK
Biochimica et biophysica acta 2015 Jul;1852(7):1477-89

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 60207-1-Ig(Galectin 3 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using LGALS3 antibody 60207-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon using 60207-1-Ig(Galectin 3 antibody) at dilution of 1:200 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon using 60207-1-Ig(Galectin 3 antibody) at dilution of 1:200 (under 40x lens)

Sample type

tissue