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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- NBP1-92690 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-92690, RRID:AB_11049950
- Product name
- Mouse Monoclonal Galectin-3 Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Isolation of human testicular cells and co-culture with embryonic stem cells.
Gaur M, Ramathal C, Reijo Pera RA, Turek PJ, John CM
Reproduction (Cambridge, England) 2018 Feb;155(2):153-166
Reproduction (Cambridge, England) 2018 Feb;155(2):153-166
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of different tissue and cell lysates using mouse mAb to galectin-3, NBP1-92690, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse brain lysate, rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at about 30kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:20,000, in red, which reveals HSP60 protein in all preparations.
