Ab104584

antibody from Aladdin Scientific

Targeting: LGALS3 GALIG, LGALS2, MAC-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab104584

Provider

Aladdin Scientific

Product name

Galectin 3 Antibody

Antibody type

Polyclonal

Description

Rabbit anti Human Galectin 3 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on.ApplicationWB: 0.1-2 µg/mLIHC: 5-20 µg/mLICC/IF: 5-20_g/mLProtein FunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 1-250


Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

Lot by Lot

Storage

Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Galectin 3 Antibody (Ab104584) - Western Blot All lanes: Galectin 3 Antibody (Ab104584) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 29 kDa Exposure time: 0.1 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Galectin 3 Antibody (Ab104584) - IF FITC staining on IF Samples: Human MCF7 cells Primary Ab: 20 μg/mL Galectin 3 Antibody (Ab104584) Second Ab: 1 μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 10 μg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Samples: Human Lung cancer Tissue Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Samples: Human Ovarian cancer Tissue Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Sample: Porcine Small intestine Tissue Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Sample: Porcine Kidney Tissue Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Sample: Porcine Spleen Tissue Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.