MA3-034

antibody from Invitrogen Antibodies

Targeting: CYP3A7 CP37, P450-HFLA

Western blot

ELISA

Immunohistochemistry

Antibody data

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Validation data

Product number: MA3-034 - Provider product page
Provider: Invitrogen Antibodies
Product name: CYP3A7 Monoclonal Antibody (F19 P2 H2)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: MA3-034 detects Cytochrome P450 3A7 from Human samples and bovine liver samples. MA3-034 has been successfully used in Western blot and Immunohistochemistry (paraffin) applications. By Western blot, this antibody detects a ~54 kDa band representing Cytochrome P450 3A7. The MA3-034 immunogen is a synthetic peptide corresponding to residues E S R D E T V S G A, conjugated to ovalbumin.
Reactivity: Human, Bovine
Host: Mouse
Isotype: IgG
Antibody clone number: F19 P2 H2
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: -20° C, Avoid Freeze/Thaw Cycles

CYP3A7 protein structure - MA3-034 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Cytochrome P450 3A7 in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Cytochrome P450 3A7 monoclonal antibody (Product # MA3-034) at a dilution of 1:80 for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Cytochrome P450 3A7 in HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Cytochrome P450 3A7 monoclonal antibody (Product # MA3-034) at a dilution of 1:80 for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Cytochrome P450 3A7 in HT29 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Cytochrome P450 3A7 monoclonal antibody (Product # MA3-034) at a dilution of 1:40 for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.