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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- GTX112784 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112784, RRID:AB_10623171
- Product name
- UCP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Organic cation transporter 3 (Oct3) is a distinct catecholamines clearance route in adipocytes mediating the beiging of white adipose tissue.
Song W, Luo Q, Zhang Y, Zhou L, Liu Y, Ma Z, Guo J, Huang Y, Cheng L, Meng Z, Li Z, Zhang B, Li S, Yee SW, Fan H, Li P, Giacomini KM, Chen L
PLoS biology 2019 Jan;17(1):e2006571
PLoS biology 2019 Jan;17(1):e2006571
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (2.5 ug of whole cell lysate) A: Interscapular brown adipose tissue 10% SDS PAGE GTX112784 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UCP1 antibody detects UCP1 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 12 % SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX112784) diluted by 1:500.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UCP1 antibody detects UCP1 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 12 % SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX112784) diluted by 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX112784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using UCP1(GTX112784) antibody at 1:250 dilution.
