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- Antibody Data
- Antigen structure
- References [3]
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- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-29575 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UCP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: mouse brown adipose. Predicted reactivity: Mouse (80%), Rat (80%), Rabbit (86%), Sheep (83%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.34 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The role of β(2) adrenergic receptor on infection development after ischaemic stroke.
Adipose depot-specific upregulation of Ucp1 or mitochondrial oxidative complex proteins are early consequences of genetic insulin reduction in mice.
Time-Dependent Molecular Responses Differ between Gastric Bypass and Dieting but Are Conserved Across Species.
Shim R, Wilson JL, Phillips SE, Lambert GW, Wen SW, Wong CHY
Brain, behavior, & immunity - health 2021 Dec;18:100393
Brain, behavior, & immunity - health 2021 Dec;18:100393
Adipose depot-specific upregulation of Ucp1 or mitochondrial oxidative complex proteins are early consequences of genetic insulin reduction in mice.
Botezelli JD, Overby P, Lindo L, Wang S, Haïda O, Lim GE, Templeman NM, Pauli JR, Johnson JD
American journal of physiology. Endocrinology and metabolism 2020 Sep 1;319(3):E529-E539
American journal of physiology. Endocrinology and metabolism 2020 Sep 1;319(3):E529-E539
Time-Dependent Molecular Responses Differ between Gastric Bypass and Dieting but Are Conserved Across Species.
Ben-Zvi D, Meoli L, Abidi WM, Nestoridi E, Panciotti C, Castillo E, Pizarro P, Shirley E, Gourash WF, Thompson CC, Munoz R, Clish CB, Anafi RC, Courcoulas AP, Stylopoulos N
Cell metabolism 2018 Aug 7;28(2):310-323.e6
Cell metabolism 2018 Aug 7;28(2):310-323.e6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UCP1 Polyclonal Antibody detects UCP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. UCP1 stained by UCP1 Polyclonal Antibody (Product # PA5-29575) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using UCP1 (Product # PA5-29575) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Evidence for systemic activation of SNS at 3 h following stroke. Noradrenaline (NA) is synthesized from dopamine by the dopamine beta-hydroxylase (DBH) enzyme ( A ). Naive mice and mice that underwent pMCAO or sham surgery and were culled and plasma was collected via cardiac puncture at 15 min ( B ), 30 min ( C ), 60 min ( D ) and 3 h ( E ) post-surgery to assess the levels of plasma NA. Data are represented as mean +- SEM, n = 3 mice per group in 15 min, 30 min and 60 min timepoints, and n = 5 for 3 h timepoints, p = 0.08, t -tests. ( F ) The protein levels of UCP-1 in the iBAT of sham-operated and post-stroke mice were assessed via Western blots at 3 h and 24 h. Data are represented as mean +- SEM, n >= 4 mice per group. * p < 0.05, t -tests. Fig. 1
