- PA5-29575 - Invitrogen Antibodies UCP1 antibody

Submitted references The role of β(2) adrenergic receptor on infection development after ischaemic stroke.

Shim R, Wilson JL, Phillips SE, Lambert GW, Wen SW, Wong CHY
Brain, behavior, & immunity - health 2021 Dec;18:100393

Adipose depot-specific upregulation of Ucp1 or mitochondrial oxidative complex proteins are early consequences of genetic insulin reduction in mice.

Botezelli JD, Overby P, Lindo L, Wang S, Haïda O, Lim GE, Templeman NM, Pauli JR, Johnson JD
American journal of physiology. Endocrinology and metabolism 2020 Sep 1;319(3):E529-E539

Time-Dependent Molecular Responses Differ between Gastric Bypass and Dieting but Are Conserved Across Species.

Ben-Zvi D, Meoli L, Abidi WM, Nestoridi E, Panciotti C, Castillo E, Pizarro P, Shirley E, Gourash WF, Thompson CC, Munoz R, Clish CB, Anafi RC, Courcoulas AP, Stylopoulos N
Cell metabolism 2018 Aug 7;28(2):310-323.e6

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of UCP1 using Mouse tissue extracts (50 µg). Samples were loaded onto a 12% SDS-PAGE gel and probed with a UCP1 polyclonal antibody (Product # PA5-29575) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of UCP1 was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a UCP1 Polyclonal Antibody (Product # PA5-29575) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using UCP1 Polyclonal Antibody (Product # PA5-29575). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 Polyclonal Antibody (Product # PA5-29575) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-UCP1 Polyclonal Antibody (Product # PA5-29575) and a 33 kDa band corresponding to UCP1 was observed only in Mouse Brown Adipose and not Mouse White Adipose. Tissue extracts (30 µg lysate) of Mouse Brown Adipose (Lane 1) and Mouse White Adipose (Lane 2) were electrophoresed using NuPAGE™ 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using UCP1 (Product # PA5-29575) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: UCP1 Polyclonal Antibody detects UCP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. UCP1 stained by UCP1 Polyclonal Antibody (Product # PA5-29575) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Evidence for systemic activation of SNS at 3 h following stroke. Noradrenaline (NA) is synthesized from dopamine by the dopamine beta-hydroxylase (DBH) enzyme ( A ). Naive mice and mice that underwent pMCAO or sham surgery and were culled and plasma was collected via cardiac puncture at 15 min ( B ), 30 min ( C ), 60 min ( D ) and 3 h ( E ) post-surgery to assess the levels of plasma NA. Data are represented as mean +- SEM, n = 3 mice per group in 15 min, 30 min and 60 min timepoints, and n = 5 for 3 h timepoints, p = 0.08, t -tests. ( F ) The protein levels of UCP-1 in the iBAT of sham-operated and post-stroke mice were assessed via Western blots at 3 h and 24 h. Data are represented as mean +- SEM, n >= 4 mice per group. * p < 0.05, t -tests. Fig. 1

PA5-29575

Antibody data