Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP14075a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP14075a, RRID:AB_10889783
- Product name
- Mouse Pdk2 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Mouse Pdk2 Antibody (N-term) (Cat. #AP14075a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Pdk2 antibody detected the Pdk2 protein (arrow).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of Pdk2 (arrow) using rabbit polyclonal Mouse Pdk2 Antibody (N-term) (Cat. #AP14075a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pdk2 gene.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Mouse Pdk2 Antibody (N-term) (Cat. #AP14075a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pdk2 antibody detected the Pdk2 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Mouse Pdk2 Antibody (N-term) (AP14075a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pdk2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50