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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-35376 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 µg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Refining the Role of Pyruvate Dehydrogenase Kinases in Glioblastoma Development.
Larrieu CM, Storevik S, Guyon J, Pagano Zottola AC, Bouchez CL, Derieppe MA, Tan TZ, Miletic H, Lorens J, Tronstad KJ, Daubon T, Røsland GV
Cancers 2022 Aug 2;14(15)
Cancers 2022 Aug 2;14(15)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDK2 in RD cell line and mouse heart, rat heart lysates. Samples were incubated with PDK2 polyclonal antibody (Product # PA5-35376). Lysates: 35 µg/lane. PDK2 protein (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PDK2 in formalin fixed and paraffin embedded skeletal muscle. Samples were incubated with PDK2 polyclonal antibody (Product # PA5-35376) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PDK2 in formalin fixed and paraffin embedded skeletal muscle. Samples were incubated with PDK2 polyclonal antibody (Product # PA5-35376) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PDK2 in Jurkat cells (bottom) compared to a negative control (top) using a PDK2 polyclonal antibody (Product # PA5-35376) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of PDK2 in Jurkat cells (bottom histogram). Samples were incubated with PDK2 polyclonal antibody (Product # PA5-35376) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
