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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-75472 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDK2 in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: L02 whole cell lysate (40 µg), Lane 3: the brain tissue lysate of mouse (40 µg), Lane 4: the brain tissue lysate of rat (40 µg), Lane 5: the liver tissue lysate of mouse (40 µg), Lane 6: the liver tissue lysate of rat (40 µg). Samples were incubated with PDK2 polyclonal antibody (Product # PA5-75472) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PDK2 in rat brain tissue lysate (sepharose bead conjugate, lane 2 and lane 3) and Nonspecific IgG Control (sepharose bead conjugate, lane 4 and lane 5). Lane 1 is 30% input. Samples were incubated with PDK2 polyclonal antibody (Product # PA5-75472) Results legends show supernatant (upward arrow) and deposition (downward arrow).
