Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN359096 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human PDK2.
- Description
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Epitope
- C-Term
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Mitochondrial mutations contribute to HIF1alpha accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma.
Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure.
Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression.
Sun W, Zhou S, Chang SS, McFate T, Verma A, Califano JA
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):476-84
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):476-84
Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure.
Richards JG, Sardella BA, Schulte PM
American journal of physiology. Regulatory, integrative and comparative physiology 2008 Sep;295(3):R979-90
American journal of physiology. Regulatory, integrative and comparative physiology 2008 Sep;295(3):R979-90
Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression.
Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA
The Journal of biological chemistry 2006 Dec 29;281(52):39897-906
The Journal of biological chemistry 2006 Dec 29;281(52):39897-906
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- (LEFT) Western blot analysis of PDK2 Antibody (C-term) in mouse intestine tissue lysate (35ug/lane). PDK2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of PDK2 (arrow) using PDK2 Antibody
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.