PA5-52890

antibody from Invitrogen Antibodies

Targeting: MGLL HU-K5, MAGL, MGL

Antibody data

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Validation data

Product number: PA5-52890 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-Monoacylglycerol Lipase Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: LVLANPESAT TFKVLAAKVL NLVLPNLSLG PIDSSVLSRN KTEVDIYNSD PLICRAGLKV CFGIQLLNAV SRVERALPKL TVPFLLLQGS ADRLCDSKGA YLLMELAKSQ DKTLKIYEGA YHVLHKELPE VTNSVFHE Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 86%.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MGLL protein structure - PA5-52890 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Monoacylglycerol Lipase in human cell line WM-115 using a Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-52890).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Monoacylglycerol Lipase in control (vector only transfected HEK293T lysate) and MGLL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-52890).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-52890) and a 35KDa band corresponding to Monoacylglycerol Lipase was observed along with uncharacterized band (*) in tissues tested. Tissue extracts (30 µg lysate) of Mouse Adipose (Lane 1), Mouse Lung (Lane 2), Rat Lung (Lane 3), Mouse liver (Lane 4) and Rat liver (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005) (http://www.jbc.org/content/272/43/27218.full.pdf).