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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunohistochemistry [3]
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- Product number
- PA5-27915 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Monoacylglycerol Lipase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, mouse adipose. Predicted reactivity: Mouse (84%), Rat (84%), Pig (91%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors.
C1q/TNF-related protein 2 (CTRP2) deletion promotes adipose tissue lipolysis and hepatic triglyceride secretion.
Crowley L, Cambuli F, Aparicio L, Shibata M, Robinson BD, Xuan S, Li W, Hibshoosh H, Loda M, Rabadan R, Shen MM
eLife 2020 Sep 11;9
eLife 2020 Sep 11;9
C1q/TNF-related protein 2 (CTRP2) deletion promotes adipose tissue lipolysis and hepatic triglyceride secretion.
Lei X, Wong GW
The Journal of biological chemistry 2019 Oct 25;294(43):15638-15649
The Journal of biological chemistry 2019 Oct 25;294(43):15638-15649
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Monoglyceride lipase using 50 µg of mouse white adipose tissue. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Monoglyceride lipase polyclonal antibody (Product # PA5-27915) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Monoglyceride lipase using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Monoglyceride lipase polyclonal antibody (Product # PA5-27915) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-27915). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-27915) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-27915) and 30kDa band corresponding to Monoacylglycerol Lipase was observed in tissues tested with lower levels in Mouse Lung. Tissue extracts (30 µg lysate) of Mouse Adipose (Lane 1) and Mouse Lung (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Monoacylglycerol Lipase Rabbit Polyclonal Antibody (Product # PA5-27915) and 30, 35kDa band corresponding to Monoacylglycerol Lipase was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A549 (Lane 3), GTL-16 (Lane 4) and U-87 MG (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Monoacylglycerol Lipase Polyclonal Antibody detects MGLL protein at nucleus on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-27915) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Monoacylglycerol Lipase Polyclonal Antibody detects Monoglyceride lipase protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-27915) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Monoacylglycerol Lipase Polyclonal Antibody detects Monoglyceride lipase protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Monoacylglycerol Lipase Polyclonal Antibody (Product # PA5-27915) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
