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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- TA336801 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal Sirtuin 1/SIRT1 Antibody (1F3)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal Sirtuin 1/SIRT1 Antibody (1F3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SIRT1
- Isotype
- IgG
- Antibody clone number
- 1F3
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: SIRT1 Antibody (1F3) [TA336801] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SIRT1 Antibody (1F3) [TA336801] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SIRT1 Antibody (1F3) [TA336801] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SIRT1 Antibody (1F3) [TA336801] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: SIRT1 Antibody (1F3) [TA336801] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: SIRT1 Antibody (1F3) [TA336801] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
- Validation comment
- FC
