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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- R33844 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Sirtuin 1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids EKPQEVQTSRNVES were used as the immunogen for this Sirtuin 1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the Sirtuin 1 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Jurkat lysate with Sirtuin 1 antibody at 0.05ug/ml. Predicted molecular weight ~80kDa but is routinely observed at 110~120kDa due to post-translational modification.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human skin with Sirtuin 1 antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
