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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00023411-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023411-M01, RRID:AB_548141
- Product name
- Mouse Monoclonal Sirtuin 1/SIRT1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SIRT1 - sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references HIPK2 restricts SIRT1 activity upon severe DNA damage by a phosphorylation-controlled mechanism.
Simvastatin inhibits cysteine-rich protein 61 expression in rheumatoid arthritis synovial fibroblasts through the regulation of sirtuin-1/FoxO3a signaling.
Remodeling of ribosomal genes in somatic cells by Xenopus egg extract.
Identification of DBC1 as a transcriptional repressor for BRCA1.
Conrad E, Polonio-Vallon T, Meister M, Matt S, Bitomsky N, Herbel C, Liebl M, Greiner V, Kriznik B, Schumacher S, Krieghoff-Henning E, Hofmann TG
Cell death and differentiation 2016 Jan;23(1):110-22
Cell death and differentiation 2016 Jan;23(1):110-22
Simvastatin inhibits cysteine-rich protein 61 expression in rheumatoid arthritis synovial fibroblasts through the regulation of sirtuin-1/FoxO3a signaling.
Kok SH, Lin LD, Hou KL, Hong CY, Chang CC, Hsiao M, Wang JH, Lai EH, Lin SK
Arthritis and rheumatism 2013 Mar;65(3):639-49
Arthritis and rheumatism 2013 Mar;65(3):639-49
Remodeling of ribosomal genes in somatic cells by Xenopus egg extract.
Østrup O, Hyttel P, Klærke DA, Collas P
Biochemical and biophysical research communications 2011 Sep 2;412(3):487-93
Biochemical and biophysical research communications 2011 Sep 2;412(3):487-93
Identification of DBC1 as a transcriptional repressor for BRCA1.
Hiraike H, Wada-Hiraike O, Nakagawa S, Koyama S, Miyamoto Y, Sone K, Tanikawa M, Tsuruga T, Nagasaka K, Matsumoto Y, Oda K, Shoji K, Fukuhara H, Saji S, Nakagawa K, Kato S, Yano T, Taketani Y
British journal of cancer 2010 Mar 16;102(6):1061-7
British journal of cancer 2010 Mar 16;102(6):1061-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - SIRT1 monoclonal antibody (M01), clone 7B7 Analysis of SIRT1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Detection limit for recombinant GST tagged SIRT1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on formalin-fixed paraffin-embedded human lateral ventricle wall. Antibody concentration 1 ug/ml.
