634301

antibody from BioLegend

Targeting: SIRT1 SIR2L1

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

634301

Provider

BioLegend

Proper citation

BioLegend Cat#634301, RRID:AB_2188350

Product name

Purified anti-SIRT1

Antibody type

Polyclonal

Antigen

Peptide mapping to the carboxy terminal domain of human SIRT1.

Description

SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors. The Poly6343 antibody has been shown to be useful for western blotting and immunoprecipitation of human SIRT1 protein.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

50 µl

Storage

The antibody solution should be stored undiluted at 4°C. DO NOT FREEZE..