MAB43391-100

antibody from R&D Systems

Targeting: GRK2 ADRBK1, BARK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB43391-100

Provider

R&D Systems

Product name

Human/Mouse/Rat GRK2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. Detects human GRK2 in direct ELISAs and Western blots.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

P25098


Isotype

IgG

Antibody clone number

2089B

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat GRK2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line, LL/2 mouse Lewis lung carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GRK2 at approximately 76 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human GRK2 by Simple Western^TM. Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK2 at approximately 80 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/ Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human GRK2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and GRK2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GRK2 at approximately 76 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.