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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MA1-2013 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRK2 Monoclonal Antibody (5D5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA1-2013 detects the beta andrenergic receptor kinase 1 in human, mouse and rat samples. MA1-2013 has been successfully used in Western blot, immunocytochemistry, immunofluoresence, immunoprecipitation, and ELISA procedures. By Western blot this antibody detects a ~30 kDa protein representing the beta andrenergeic receptor kinase 1 in human HeLa cells. In immunocytochemistry procedures MA1-2013 recognizes the beta andrenergic receptor kinase 1 in HeLa, Hek-293 and 3T3 cells. Immunofluoresence in HeLa cells yeilds predominantely cytoplasmic membrane associated staining with some staining in the ruffles. The MA1-2013 immunogen is recombinant beta adrenergic receptor kinase 1.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5D5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Cardioprotective effects of exercise training on myofilament calcium activation in ovariectomized rats.
Bupha-Intr T, Wattanapermpool J
Journal of applied physiology (Bethesda, Md. : 1985) 2004 May;96(5):1755-60
Journal of applied physiology (Bethesda, Md. : 1985) 2004 May;96(5):1755-60
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluoresence staining of beta adrenergic receptor kinase 1 in HeLa cells using Product # MA1-2013.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GRK2 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRK2 (5D5) Mouse Monoclonal Antibody (Product # MA1-2013) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized human heart tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing beta Adrenergic Receptor Kinase 1 (Product # MA1-2013) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
