MA5-15840

antibody from Invitrogen Antibodies

Targeting: GRK2 ADRBK1, BARK1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15840

Provider

Invitrogen Antibodies

Product name

GRK2 Monoclonal Antibody (3F8)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15840 targets GRK2 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples. The MA5-15840 immunogen is purified recombinant fragment of human GRK2 expressed in E. Coli. . MA5-15840 detects GRK2 which has a predicted molecular weight of approximately 80kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3F8

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

GRK2 mediates β-arrestin interactions with 5-HT(2) receptors for JC polyomavirus endocytosis.

Mayberry CL, Wilczek MP, Fong TM, Nichols SL, Maginnis MS
Journal of virology 2021 Mar 10;95(7)

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A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer.

Buchholz M, Honstein T, Kirchhoff S, Kreider R, Schmidt H, Sipos B, Gress TM
PloS one 2015;10(4):e0122946

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH/3T3 cells using GRK2 monoclonal antibody (Product # MA5-15840) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GRK2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRK2 Monoclonal Antibody (3F8) (Product # MA5-15840) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 5 ADRBK1 overexpression promotes growth in human PDAC. A: Box-and-whisker plot showing ADRBK1 mRNA expression in primary human pancreatic tumor tissue samples, chronic pancreatitis and normal pancreas as analyzed by quantitative realtime reverse transcription PCR (qRT-PCR). Expression was normalized to ribosomal protein, large, P0 (RPLP0) mRNA levels. Data in Fig. represent median and 2nd and 3rd quartiles (boxes) as well as minimum and maximum values (whiskers). CP = Chronic Pancreatitis. ** p