Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- ABIN2616298 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Angiopoietin-Like 4 (ANGPTL4) (AA 79-101) antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 79-101
- Vial size
- 100 μg
- Storage
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles.
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- CENP-A phospho Ser18 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of CENP-A phospho Ser18 antibody. Peptides corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:750. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: CENP-A phospho Ser 16 and phospho Ser18. Lane 2: CENP-A unmodified. Lane 3: CENP-A phospho Ser 16. Lane 4: CENP-A phospho Ser 18.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- CENP-A phospho Ser18 antibody (pAb) tested by Western blot. Top: Recombinant CENP-A (20 ng) was probed with CENP-A phospho Ser18 antibody (1:100 dilution) (A) or with anti-centromere antibody (ACA) (B). CENP-A phospho Ser18 antibody does not detect recombinant CENP-A. Bottom: HeLa nuclear extract (20 μg) was probed with CENP-A phospho Ser18 antibody (1:250 dilution). Lane 1: CENP-A phospho-Ser18 antibody. Lane 2: anti-centromere antibody (ACA).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- CENP-A phospho Ser18 antibody (pAb) tested by immunofluorescence. Detection of CENP-A phospho Ser18 by immunofluorescence. HeLa cells were stained with CENP-A phospho Ser18 antibody at a 1:100 dilution. Green: CENP-A phospho Ser18 antibody (pAb) Blue: DAPI