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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- ABIN2616301 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Angiopoietin-Like 4 (ANGPTL4) (Fibrinogen-like Domain) antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified from tissue culture supernatant
- Reactivity
- Human
- Host
- Mouse
- Epitope
- Fibrinogen-like Domain
- Isotype
- IgG
- Antibody clone number
- Kairos-1
- Vial size
- 100 μg
- Storage
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AIB1 antibody (pAb) tested by Western blot. Detection of AIB1 by Western blot. Lane 1: HeLa whole-cell extract (30 μg). Lane 2: Whole cell extract (30 μg) of MCF-7 cells. Both probed with AIB1 antibody at a 1:500 dilution.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AIB1 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 μl of AIB1 / SRC-3 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the pS2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
