PA5-26216

antibody from Invitrogen Antibodies

Targeting: ANGPTL4 ARP4, FIAF, HFARP, NL2, PGAR, pp1158

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-26216

Provider

Invitrogen Antibodies

Product name

ANGPTL4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Molecular Fingerprint for Terminal Abdominal Aortic Aneurysm Disease.

Gäbel G, Northoff BH, Weinzierl I, Ludwig S, Hinterseher I, Wilfert W, Teupser D, Doderer SA, Bergert H, Schönleben F, Lindeman JHN, Holdt LM
Journal of the American Heart Association 2017 Nov 30;6(12)

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ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression.

Aryal B, Rotllan N, Araldi E, Ramírez CM, He S, Chousterman BG, Fenn AM, Wanschel A, Madrigal-Matute J, Warrier N, Martín-Ventura JL, Swirski FK, Suárez Y, Fernández-Hernando C
Nature communications 2016 Jul 27;7:12313

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using an ANGPTL4 polyclonal antibody (Product # PA5-26216) in mouse liver tissue lysates (35 µg per lane).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HepG2 cells using an ANGPTL4 polyclonal antibody (Product # PA5-26216) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Results of the immunohistochemistry. Staining for protein products of our candidate genes confirmed presence in the aneurysmatic wall. ANGPTL 4, HILPDA , SRPX 2, and LOX are localized in pericytes of microvessels in the medial-adventitial border zone. Staining for SRPX 2 is diffuse in the media and also observed in adipocytes of the adventitia. Demonstrated are representative sections from a 55-mm stable abdominal aortic aneurysm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 ANGPTL4 is expressed in macrophages accumulated in atherosclerotic plaques. ( a ) Heat map representation of gene expression from microarray data comparing mouse peritoneal macrophages incubated with or without Ac-LDL (120 mug ml -1 ) for 24 h. Left panel shows upregulated genes ( P =1.4) and right panel shows downregulated genes ( P =2.08) upon Ac-LDL treatment compared with non-treated cells. Samples were analysed in triplicate. ( b ) qRT-PCR validation of selected genes upregulated in the microarray in mouse peritoneal macrophages treated with or without Ac-LDL for 24 h. qRT-PCR analysis of Angptl4 expression levels in macrophages ( c , left) and whole aorta ( c , right) from WT and Ldlr -/- mice fed a WD (left) ( n =4 per group), and in human peripheral blood mononuclear cells ( d ) treated with or without Ac-LDL (120 mug ml -1 ) for 24 h. Abca1 and Hmgcr genes were used as control genes for cholesterol loading and Cd68 was used as a marker for macrophages. All data represent the mean+-s.e.m. and are representative of three experiments in duplicate; * P