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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00009443-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009443-M01, RRID:AB_538004
- Product name
- Mouse Monoclonal MED7 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CRSP9 - cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED7 Antibody (3E7) [H00009443-M01] - Analysis of CRSP9 over-expressed 293 cell line, cotransfected with CRSP9 Validated Chimera RNAi ( Cat # H00009443-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRSP9 monoclonal antibody (M01), clone 3E7 (Cat # H00009443-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED7 Antibody (3E7) [H00009443-M01] - Analysis of CRSP9 expression in transfected 293T cell line by CRSP9 monoclonal antibody (M01), clone 3E7.Lane 1: CRSP9 transfected lysate(27.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED7 Antibody (3E7) [H00009443-M01] - CRSP9 monoclonal antibody (M01), clone 3E7 Analysis of CRSP9 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: MED7 Antibody (3E7) [H00009443-M01] - Detection limit for recombinant GST tagged CRSP9 is approximately 0.3ng/ml as a capture antibody.
